Inhibition of the central extended amygdala by loud noise and restraint stress
Inhibition of the central extended amygdala by loud noise and restraint stress
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DOI：
10.1111/j.1460-9568.2005.03865.x
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发表时间：
2005-01-01
影响因子：
3.4
通讯作者：
Campeau, S
中科院分区：
文献类型：
--
作者：
Day, HEW;Nebel, S;Campeau, S
众所周知，杏仁核中央核（CEA）参与对压力、恐惧和焦虑的反应。许多研究使用c-fos表达来绘制大脑对过程应激的反应，但奇怪的是，CEA通常不是高度激活的。我们之前的研究表明，暴露于新的环境与家庭环境相比，安非他明诱导的侧CEA （CEAl）和终纹床核（BSTov）卵形核的激活会减少。这与过程应激抑制这些核中的神经元的观点是一致的。我们已经通过将大鼠暴露在噪音中来测试这一假设，从无压力到有压力，或在约束条件下，在远程注射安非他明后立即，2mg /kg i.p或白细胞介素-1 β (il -1 β) 0.5杯/kg i.p（用于获得c-fos mRNA的水平，以测量抑制作用）。与我们的假设一致，与对照组相比，在大噪声或约束应激条件下，安非他明或il -1 β诱导的CEAl和BSTov中的c-fos和zif-268 mRNA显著降低。这种抑制不需要应激诱导的皮质酮升高，因为在用低剂量皮质酮替代肾上腺切除的动物中数据相似。由于CEAl和BSTov都具有高γ -氨基丁酸（GABA）能并投射到内侧CEA (CEAm)，它们的抑制可能导致CEAm输入增加。由于CEAm是杏仁核的主要输出核，这可能对控制过程应激反应的神经回路产生重要影响。
It is well established that the central nucleus of the amygdala (CEA) is involved in responses to stress, fear and anxiety. Many studies have used c-fos expression to map the brain's response to processive stress, but curiously the CEA generally is not highly activated. We have previously shown that exposure to a novel vs. home environment reduces amphetamine-induced activation of the lateral CEA (CEAl) and the oval nucleus of the bed nucleus of the stria terminalis (BSTov). This is consistent with the idea that processive stress inhibits neurons in these nuclei. We have tested this hypothesis by exposing rats to noise, at a range of intensities from non-stressful to stressful, or to restraint conditions, immediately after a remote injection of amphetamine, 2 mg/kg i.p., or interleukin-1beta (IL-1beta) 0.5 mug/kg i.p. (used to obtain a level of c-fos mRNA against which to measure inhibition). In keeping with our hypothesis, amphetamine- or IL-1beta-induced c-fos and zif-268 mRNA were significantly decreased in the CEAl and BSTov under conditions of loud noise or restraint stress compared with control conditions. This inhibition does not require a stress-induced rise in corticosterone because data were similar in animals that had been adrenalectomized with a low-dose corticosterone replacement. As both the CEAl and BSTov are highly gamma-aminobutyric acid (GABA) -ergic and project to the medial CEA (CEAm), their inhibition potentially causes an increased input to the CEAm. As the CEAm is a major output nucleus of the amygdala, this could have important consequences within the neural circuitry controlling responses to processive stress.