[Establishment of a new human endometrial adenocarcinoma cell line, Ishikawa cells, containing estrogen and progesterone receptors].
[Establishment of a new human endometrial adenocarcinoma cell line, Ishikawa cells, containing estrogen and progesterone receptors].
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发表时间：
1985-07
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通讯作者：
M. Nishida;K. Kasahara;M. Kaneko;H. Iwasaki;K. Hayashi
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作者：
M. Nishida;K. Kasahara;M. Kaneko;H. Iwasaki;K. Hayashi
一种新的人子宫内膜腺癌细胞系Ishikawa细胞系是从一位39岁妇女的子宫内膜腺癌中建立起来的，并已在体外培养了3年多。发现这些细胞以镶嵌的方式形成单层，并倾向于堆积。计算出第9、40、50代种群倍增时间分别为36、29、27小时。细胞的模式染色体数目落在二倍体范围内。裸鼠体内诱导的肿瘤组织学显示，它是一种分化良好的腺癌，与原始的人类肿瘤非常相似。雌激素受体和孕激素受体不仅存在于裸鼠诱发的肿瘤中，也存在于体外培养的细胞中。从细胞在无雌激素的培养液中保持生长的事实来看，细胞似乎不依赖雌激素。
A new human endometrial adenocarcinoma cell line, Ishikawa cells, was established from an endometrial adenocarcinoma from a 39-year-old woman and has been maintained in vitro for more than 3 years. The cells were found to form a monolayer in a mosaic fashion and to tend to pile up. Population doubling time was calculated to be about 36, 29 and 27 hours at the 9th, 40th and 50th generations, respectively. The modal chromosomal number of the cells fell in a diploid range. Histology of the tumor induced in athymic nude mice showed it to be a well differentiated adenocarcinoma which closely resembled the original human tumor. Estrogen receptor and progesterone receptor were demonstrated to occur not only in the induced tumor in athymic nude mice but also in in vitro culture cells. From the fact that the cell growth was maintained in an estrogen-free medium, it appeared that the cells had no estrogen dependency.