Transplantation of Zebrafish Cells by Conventional Pneumatic Microinjector
Transplantation of Zebrafish Cells by Conventional Pneumatic Microinjector
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传统气动显微注射器移植斑马鱼细胞
DOI：
10.1089/zeb.2017.1495
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发表时间：
2018-02-01
期刊：
影响因子：
2
通讯作者：
Shi, De-Li
中科院分区：
文献类型：
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作者：
Shao, Ming;Cheng, Xiao-Ning;Shi, De-Li
通过移植产生嵌合斑马鱼对于发育、干细胞和癌症生物学中的活体成像以及回答细胞如何获得、保持和/或改变其命运的问题是非常有用的。然而，由于技术上的挑战，移植方法的使用仍然非常有限的斑马鱼社区。在这项研究中，我们表明，这种细胞移植操作可以很容易地实现，通过使用传统的气动显微注射器通常用于显微注射。与以前发表的需要额外移植设备的方案相比，这种替代移植方法效果良好，但需要更简单的实验装置，并且更容易为所有研究者所用。
Generating chimeric zebrafish by transplantation is extremely useful for live imaging in developmental, stem cell, and cancer biology, and to answer the questions of how cells acquire, keep, and/or change their fate. However, as it is technically challenging, the use of transplantation approach remains very limited by the zebrafish community. In this study, we show that this cell grafting operation can be easily achieved by using a conventional pneumatic microinjector normally used for microinjections. Compared with previously published protocols, which need additional transplantation apparatus, this alternative transplantation method works well, but needs a simpler experimental setup, and is more accessible to all investigators.