The intron-containing genome of the lytic Pseudomonas phage LUZ24 resembles the temperate phage PaP3
The intron-containing genome of the lytic Pseudomonas phage LUZ24 resembles the temperate phage PaP3
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DOI：
10.1016/j.virol.2008.04.038
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发表时间：
2008-08-01
期刊：
影响因子：
3.7
通讯作者：
Lavigne, Rob
中科院分区：
文献类型：
--
作者：
Ceyssens, Pieter-Jan;Hertveldt, Kirsten;Lavigne, Rob
从医院污水中分离出毒性铜绿假单胞菌噬菌体LUZ 24（45，625 bp）。它属于短尾病毒科，携带一个双向转录的dsDNA基因组，由两个184 bp的直接末端重复序列描绘。体外转录分析鉴定了7个sigma（70）启动子，揭示了在晚期基因组区域中偏向于更强的启动子强度。逆转录证明了嵌入DNA聚合酶基因内的668 bp I组内含子的体内剪接。使用质谱法，九个结构蛋白被鉴定为噬菌体颗粒的一部分。LUZ 24的裂解特性针对其基因组内容进行评价，其显示与温和噬菌体PaP3的总体71%的序列相似性。(C)2008年爱思唯尔公司All rights reserved.
The virulent Pseudomonas aeruginosa bacteriophage LUZ24 (45,625 bp) was isolated from hospital sewage. It belongs to the family of the Podoviridae, and carries a bidirectionally transcribed dsDNA genome delineated by two direct terminal repeats of 184 bp. In vitro transcriptional analysis identified seven sigma(70) promoters, revealing a bias towards stronger promoter strength in the late genomic region. Reverse transcription demonstrated in vivo splicing of a 668 bp Group I intron embedded inside the DNA polymerase gene. Using mass spectrometry, nine structural proteins were identified as part of the phage particle. The lytic characteristics of LUZ24 are evaluated against its genomic content, which displays an overall 71% sequence similarity to the temperate phage PaP3. (C) 2008 Elsevier Inc. All rights reserved.