CLINICALLY USEFUL METHOD FOR ISOLATION OF RESPIRATORY SYNCYTIAL VIRUS
CLINICALLY USEFUL METHOD FOR ISOLATION OF RESPIRATORY SYNCYTIAL VIRUS
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DOI：
10.1093/infdis/131.1.1
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发表时间：
1975-01-01
影响因子：
6.4
通讯作者：
DOUGLAS, RG
中科院分区：
文献类型：
--
作者：
HALL, CB;DOUGLAS, RG
本文报道了一种分离呼吸道合胞病毒(RSV)的简便方法，该方法相对快速，病毒回收率高。将具有高滴度病毒的鼻分泌物样本在床边接种到敏感细胞系上，以避免由于病毒的不稳定而失去病毒感染性。在一次RSV暴发期间，通过这种方法从所有住院的下呼吸道疾病患儿中获得了病毒样本。在这45名儿童中，检出呼吸道合胞病毒或甲型流感病毒的比例为%。87%的肺炎患者体内分离到呼吸道合胞病毒。通过常规鼻咽拭子同时获得的样本中，RSV的检出频率降低了60%。使用床边鼻腔冲洗方法可以更快地识别RSV的细胞病变效应，平均在4天内完成。因此，当这些信息对患者的病情管理仍然有用时，临床医生就可以使用这些信息。
A simple method for the isolation of respiratory syncytial virus (RSV) is reported; it is relatively rapid and results in a high frequency of recovery of virus. A nasal secretion specimen with high titers of virus is inoculated at the bedside onto susceptible cell lines to avoid loss of viral infectivity due to lability of the virus. During an outbreak of RSV, viral specimens were obtained by this method from all young children admitted to the hospital with lower respiratory tract disease. RSV or influenza A virus was recovered from 89% of these 45 children. RSV was isolated from 87% of those with pneumonia. RSV was recovered 60% less often from specimens obtained simultaneously by conventional nasopharyngeal swabs. Identification of RSV cytopathic effect was more rapid with use of the bedside nasal wash method and was accomplished in an average of four days. Hence, this information was available to the clinician when it was still useful in the management of the patient's illness.