Respiratory systems and cytochromes in Campylobacter fetus subsp. intestinalis.
Respiratory systems and cytochromes in Campylobacter fetus subsp. intestinalis.
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胎儿弯曲杆菌亚种的呼吸系统和细胞色素。
DOI：
10.1128/jb.144.3.917-922.1980
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发表时间：
1980
影响因子：
3.2
通讯作者：
Lascelles,J
中科院分区：
文献类型：
--
作者：
Harvey,S;Lascelles,J
胎儿弯曲杆菌亚种的细胞悬液。在甲酸盐、琥珀酸盐和DL-乳酸盐存在下，在复杂介质中微需氧生长的膜消耗氧气，并且膜具有相应的脱氢酶活性。细胞和细胞膜还具有抗坏血酸-N，N，N ′，N ′-四甲基-对苯二胺氧化酶活性，这是氰化物敏感的。对氯汞苯磺酸能抑制膜中延胡索酸还原酶的活性，该酶可能与琥珀酸脱氢酶的活性有关。细胞色素C是占主导地位的膜，这种色素的主要比例表现出一氧化碳结合光谱。抗坏血酸-N，N，N '，N'-四甲基-对苯二胺也能还原细胞色素c总量的60%。琥珀酸在厌氧条件下减少了类似比例的膜细胞色素c，而甲酸减少了90%以上的总细胞色素在这些条件下。2-庚基-4-羟基喹啉-N-氧化物抑制琥珀酸盐还原细胞色素c，使细胞色素B的还原谱明显。抑制剂延迟还原细胞色素c与甲酸盐，但最终的还原水平不受影响。我们的结论是，呼吸链包括低和高电位形式的细胞色素c和B;一氧化碳结合形式的细胞色素c可能作为一个终端氧化酶的功能。
Cell suspensions of Campylobacter fetus subsp. intestinalis grown microaerophilically in complex media consumed oxygen in the presence of formate, succinate, and DL-lactate, and membranes had the corresponding dehydrogenase activities. The cells and membranes also had ascorbate-N,N,N',N'-tetramethyl-p-phenylenediamine oxidase activity which was cyanide sensitive. The fumarate reductase activity in the membranes was inhibited by p-chloromercuriphenylsulfonate, and this enzyme was probably responsible for the succinate dehydrogenase activity. Cytochrome c was predominant in the membranes, and a major proportion of this pigment exhibited a carbon monoxide-binding spectrum. Approximately 60% of the total membrane cytochrome c, measured with dithionite as the reductant, was also reduced by ascorbate-N,N,N',N'-tetramethyl-p-phenylenediamine. A similar proportion of the membrane cytochrome c was reduced by succinate under anaerobic conditions, whereas formate reduced more than 90% of the total cytochrome under these conditions. 2-Heptyl-4-hydroxyquinoline-N-oxide inhibited reduction of cytochrome c with succinate, and the reduced spectrum of cytochrome b became evident. The inhibitor delayed reduction of cytochrome c with formate, but the final level of reduction was unaffected. We conclude that the respiratory chain includes low- and high-potential forms of cytochromes c and b; the carbon monoxide-binding form of cytochrome c might function as a terminal oxidase.