Virus Attachment to Transparent Exopolymeric Particles along Trophic Gradients in the Southwestern Lagoon of New Caledonia
Virus Attachment to Transparent Exopolymeric Particles along Trophic Gradients in the Southwestern Lagoon of New Caledonia
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新喀里多尼亚西南部泻湖沿营养梯度的透明外聚合物颗粒上的病毒附着
DOI：
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发表时间：
2007
影响因子：
4.4
通讯作者：
M. Weinbauer
中科院分区：
文献类型：
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作者：
X. Mari;M. Kerros;M. Weinbauer
病毒在透明外聚颗粒（TEP）等有机聚集体上的研究尚不充分。在新喀里多尼亚西南部泻湖，通过测定磁分离TEP后去除的病毒比例，沿营养梯度评估TEP附着病毒的数量。为了磁性分离TEP， TEP在沿着营养梯度收集的亚微米前体的磁珠存在下形成。在磁场作用下，将tep -病毒混合聚集体从溶液中去除。大多数站点与新形成的TEP相关的病毒百分比平均为8%（范围为3%至13%），但在一个以其水质量更新率低为特征的海湾中较高（约30%）。根据公式N = 100d1.60和N = 230d1.75，在短时间（即<40天）和长时间（即40天）存在的水体中，附着在TEP上的病毒数N随TEP大小（d[微米]）的变化而变化。这两种关系表明病毒丰度随TEP大小而降低，它们表明在水中停留时间影响病毒密度和病毒-细菌在聚集体内的相互作用。我们的数据表明，在水质量更新率低的地区，附着在TEP上的病毒比例最高，有时可能构成总病毒丰度的很大一部分。由于TEP上的病毒与宿主之间的距离较小，这些颗粒可能是病毒感染的热点。
ABSTRACT Viruses on organic aggregates such as transparent exopolymeric particles (TEP) are not well investigated. The number of TEP-attached viruses was assessed along trophic gradients in the southwestern lagoon of New Caledonia by determining the fraction of viruses removed after magnetic isolation of TEP. In order to isolate TEP magnetically, TEP were formed in the presence of magnetic beads from submicrometer precursors collected along the trophic gradients. The mixed aggregates of TEP-beads-viruses were removed from solution with a magnetic field. The percentage of viruses associated with newly formed TEP averaged 8% (range, 3 to 13%) for most of the stations but was higher (ca. 30%) in one bay characterized by the low renewal rate of its water mass. The number of viruses (N) attached to TEP varied as a function of TEP size (d [in micrometers]) according to the formulas N = 100d1.60 and N = 230d1.75, respectively, for TEP occurring in water masses with short (i.e., <40 days) and long (i.e., >40 days) residence times. These two relationships imply that viral abundance decreases with TEP size, and they indicate that water residence time influences viral density and virus-bacterium interactions within aggregates. Our data suggest that the fraction of viruses attached to TEP is highest in areas characterized by a low renewal rate of the water mass and can constitute at times a significant fraction of total virus abundance. Due to the small distance between viruses and hosts on TEP, these particles may be hot spots for viral infection.