Evaluation of photodynamic therapy (PDT) procedures using microfluidic system
Evaluation of photodynamic therapy (PDT) procedures using microfluidic system
复制标题
DOI：
10.1016/j.aca.2010.10.005
复制
发表时间：
2011-01-10
影响因子：
6.2
通讯作者：
Brzozka, Zbigniew
中科院分区：
文献类型：
--
作者：
Jedrych, Elzbieta;Pawlicka, Zuzanna;Brzozka, Zbigniew
提出了一种用于评价光动力治疗效率的PDMS/玻璃微流控混合系统。以5-氨基乙酰丙酸(ALA)为光敏剂前驱体。本文提出的微器件的几何结构使得能够在一次检测中检测不同浓度的光敏剂。光动力疗法(PDT)后24小时测定A549细胞的存活率(光敏剂在癌细胞中积累，波长为625 nm)。目前的结果证实了在微尺度上进行体外光动力学治疗过程的可能性，以及评估其有效性的可能性。此外，由于在同一芯片上执行了两个相同的微结构，该芯片可以用于同时检测各种细胞系(癌细胞和正常细胞)或各种光敏剂。(C)2010爱思唯尔B.V.保留所有权利。
A hybrid PDMS/glass microfluidic system for evaluation of the efficiency of photodynamic therapy is presented. 5-aminolevulinic acid (ALA) was used as a precursor of photosensitizer. The geometry of the microdevice presented in this paper enables to test different concentrations of the photosensitizer in a single assay. The viability of the A549 cells was determined 24h after PDT procedure (irradiation with light which induced a photosensitizer accumulated in carcinoma cells, lambda = 625 nm). The presented results confirmed the possibility to perform the photodynamic therapy process in vitro in microscale and the possibility to assess its effectiveness. Moreover, because two identical microstructures on a single chip were performed, the microchip can be used for examination simultaneously various cell lines (carcinoma and normal) or various photosensitizers. (C) 2010 Elsevier B.V. All rights reserved.