DISTRIBUTION AND CHARACTERIZATION OF IMMUNOREACTIVE ADRENOMEDULLIN IN HUMAN TISSUE AND PLASMA
DISTRIBUTION AND CHARACTERIZATION OF IMMUNOREACTIVE ADRENOMEDULLIN IN HUMAN TISSUE AND PLASMA
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DOI：
10.1016/0014-5793(94)80106-1
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发表时间：
1994-01-24
期刊：
影响因子：
3.5
通讯作者：
ETO, T
中科院分区：
文献类型：
--
作者：
ICHIKI, Y;KITAMURA, K;ETO, T
建立了一种特异、灵敏的肾上腺髓质素放射免疫分析方法，并研究了肾上腺髓质素在人体组织中的分布和特性。放射免疫测定特异性识别其羧基末端区域，在11 fmol/管下观察到放射性碘标记肾上腺髓质素（40-52）NH 2结合的半数最大抑制。免疫反应性肾上腺髓质素在肾上腺髓质中丰富（47.7 +/- 26.1 fmol/mg，平均值+/- S.D.）并且在所有检查的组织中普遍存在。三名正常人肾上腺髓质素的平均血浆浓度为17.2 ± 6.4 pg/ml（平均值± S.D.）。用反相高效液相色谱法结合放射免疫分析，发现肾上腺髓质、心房和肺中免疫反应性最强的肾上腺髓质素是肾上腺髓质素（1-52）NH 2。
A specific and sensitive radioimmunoassay for human adrenomedullin has been developed and distribution and characterization of immunoreactive adrenomedullin in human tissue were investigated. The radioimmunoassay specifically recognizes its carboxyterminal region and half maximal inhibition of binding of radioiodinated adrenomedullin(40-52)NH2 was observed at 11 fmol/tube. Immunoreactive adrenomedullin was abundant in adrenal medulla (47.7 +/- 26.1 fmol/mg, mean +/- S.D.) and was ubiquitously found in all tissue examined. The mean plasma concentration of adrenomedullin in three normal individuals was 17.2 +/- 6.4 pg/ml (mean +/- S.D.). By analysis with reverse-phase high-performance liquid chromatography coupled with the radioimmunoassay, most immunoreactive adrenomedullin in the adrenal medulla, atrium and lung was found to be adrenomedullin(1-52)NH2.