Polarity orientation of microtubules utilizing a dynein-based gliding assay
Polarity orientation of microtubules utilizing a dynein-based gliding assay
复制标题
利用基于动力蛋白的滑动测定进行微管的极性定向
作者：
R. Yokokawa;T. Murakami;T. Sugie;T. Kon
将马达蛋白动力蛋白引入纳米转运系统。我们根据微管的极性将其定向，并基于动力蛋白-微管滑动分析系统将其固定化。该系统实现了驱动蛋白包被的微珠的单向运输。与传统的基于驱动蛋白的取向系统相反，基于动力蛋白的系统允许微管的反向运动，导致微管极性的取向反转，从而导致驱动蛋白包被的微珠的反向运输。这种基于驱动蛋白和动力蛋白的组合系统构成了促进微管双向取向和纳米流体系统中货物运输的新手段。
The motor protein dynein was introduced into a nanotransport system. We oriented microtubules by their polarity, and immobilized them based on a dynein–microtubule gliding assay system. This system achieved unidirectional transport of kinesin-coated microbeads. In contrast to conventional kinesin-based orientation systems, the dynein-based system allowed the reverse motion of microtubules, resulting in an inversion of the orientation of microtubule polarity and thus reverse transport of kinesin-coated microbeads. This combined kinesin- and dynein-based system constitutes a new means to facilitate the bidirectional orientation of microtubules and transport of cargos in a nanofluidic system.