Structure of the mouse activin receptor type II gene.
Structure of the mouse activin receptor type II gene.
复制标题
小鼠激活素受体 II 型基因的结构。
DOI：
10.1016/s0006-291x(05)81000-9
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发表时间：
1992
影响因子：
3.1
通讯作者：
Bradley,A
中科院分区：
文献类型：
--
作者：
Matzuk,MM;Bradley,A
激活素受体基因结构的知识对于我们理解激活素、抑制素及其受体在发育和生理过程中的作用至关重要。II型激活素受体（ActRc）已被证明是一种跨膜蛋白，具有丝氨酸/苏氨酸激酶活性。以人ActRc II型cDNA为探针，从小鼠129SvE基因组文库中分离出18个重叠的λ克隆，其中包含小鼠ActRc II型基因的部分。通过外显子、外显子-内含子边界、5′和3′非翻译区序列分析以及小鼠基因组DNA的Southern blot分析，建立小鼠ActRc II型基因的结构。小鼠ActRc II型基因由11个外显子编码，跨越bb1066千碱基。两个大内含子（内含子1和内含子4）占基因大小的大部分，并被发现描绘编码激活素受体重要结构域的外显子。对该基因5 '区的分析揭示了几个可能的转录因子结合位点，这些位点可能对该基因的复杂转录调控很重要。
Knowledge of the structures of the activin receptor genes is crucial to our understanding of the role of the activins, inhibins, and their receptors in developmental and physiological processes. The type II activin receptor (ActRc) has been shown to be a transmembrane protein with putative serine/threonine kinase activity. Using a human ActRc type II cDNA as a probe, 18 overlapping λ clones containing portions of the mouse ActRc type II gene were isolated from a mouse 129SvE genomic library. Sequence analysis of the exons, exon-intron boundaries, and 5′ and 3′ nontranslated regions as well as Southern blot analysis of mouse genomic DNA were used to establish the structure of the mouse ActRc type II gene. The mouse ActRc type II gene is encoded by 11 exons and spans >66 kilobases. Two large introns (introns 1 and 4) contribute the majority of the gene size and are found to delineate exons which encode important domains of the activin receptor. Analysis of the 5′ region of the gene reveals several putative transcription factor binding sites which may be important for the complex transcriptional regulation of this gene.