Characterization of the effects of n-butanol on the cell envelope of E-coli
Characterization of the effects of n-butanol on the cell envelope of E-coli
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DOI：
10.1007/s00253-016-7771-6
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发表时间：
2016-11-01
影响因子：
5
通讯作者：
French, Chris E.
中科院分区：
文献类型：
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作者：
Fletcher, Eugene;Pilizota, Teuta;French, Chris E.
生物燃料酒精对其生物合成所使用的微生物宿主有严重的影响，从而限制了生物转化的生产率。细胞膜是受影响最大的结构之一，特别是在生物合成过程中，生物燃料的外部浓度上升。对细胞被膜的破坏可能会产生严重的后果，如细胞进出运输的障碍；然而，丁醇引起的被膜损害的性质尚未得到很好的描述。在本研究中，研究了正丁醇对大肠杆菌细胞膜的影响。用酶法和荧光法，我们观察到1%v/v正丁醇可引起大肠杆菌外膜脂多糖的释放，并引起外膜和内膜的“渗漏”。较高浓度的正丁醇，在2-10%(v/v)的范围内，通过典型的气泡观察到通过肽聚糖的内膜突出。这一发现表明，合理设计耐丁醇细菌菌株的策略应该考虑到细胞被膜的所有成分。
Biofuel alcohols have severe consequences on the microbial hosts used in their biosynthesis, which limits the productivity of the bioconversion. The cell envelope is one of the most strongly affected structures, in particular, as the external concentration of biofuels rises during biosynthesis. Damage to the cell envelope can have severe consequences, such as impairment of transport into and out of the cell; however, the nature of butanol-induced envelope damage has not been well characterized. In the present study, the effects of n-butanol on the cell envelope of Escherichia coli were investigated. Using enzyme and fluorescence-based assays, we observed that 1 % v/v n-butanol resulted in the release of lipopolysaccharides from the outer membrane of E. coli and caused 'leakiness' in both outer and inner membranes. Higher concentrations of n-butanol, within the range of 2-10 % (v/v), resulted in inner membrane protrusion through the peptidoglycan observed by characteristic blebs. The findings suggest that strategies for rational engineering of butanol-tolerant bacterial strains should take into account all components of the cell envelope.