Collection of peripheral blood stem cells mobilized by high-dose Ara-C plus VP-16 or aclarubicin followed by recombinant human granulocyte-colony stimulating factor.
Collection of peripheral blood stem cells mobilized by high-dose Ara-C plus VP-16 or aclarubicin followed by recombinant human granulocyte-colony stimulating factor.
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收集通过高剂量 Ara-C 加 VP-16 或阿柔比星动员的外周血干细胞，然后加入重组人粒细胞集落刺激因子。
DOI：
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发表时间：
1992
影响因子：
4.8
通讯作者：
S. Murakami
中科院分区：
文献类型：
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作者：
C. Shimazaki;N. Oku;E. Ashihara;K. Okawa;H. Goto;T. Inaba;K. Ito;N. Fujita;H. Tsuji;S. Murakami
我们开发了一种有效的方法来收获大量的外周血干细胞（PBSC）用于自体移植。20例恶性血液病患者接受高剂量阿糖胞苷（12 g/m2）和VP-16/阿糖胞苷治疗，随后给予rhG-CSF（50 μ g/m2）。采用免疫磁珠法检测外周血中CD34阳性干细胞数，确定外周血干细胞采集的最佳时间。用CS-3000血细胞分离机收集PBSC。每次单采时处理的总血量为7000 - 9000 ml。在这些条件下，20例患者共进行了64次单采术。每次单采收获的单核细胞和CFU-GM的平均数量分别为4.1 × 10（8）/kg和110 × 10（4）/kg。在20名患者中，15名患者通过单次单采可以获得足够植入的CFU-GM数量（> 30 × 10（4）/kg）。迄今为止，已有11例患者接受了PBSC移植，并获得了快速的造血恢复。中性粒细胞恢复到0.5 × 10（9）/l以上的中位时间为10天，血小板恢复到50 × 10（9）/l的中位时间为11天。这种收获大量PBSC的方法允许在具有化学放射敏感性肿瘤的患者中进行更安全的自体移植，并且适用于老年患者。
We developed an effective method for harvesting large numbers of peripheral blood stem cells (PBSC) for use in autotransplantation. Twenty patients with hematological malignancies were treated with high doses of Ara-C (12 g/m2) and VP-16/aclarubicin followed by administration of rhG-CSF (50 micrograms/m2). The optimal time for starting PBSC collection was determined by monitoring the CD34-positive stem cells in blood using immunomagnetic beads. PBSC were collected with a CS-3000 blood cell separator. A total blood volume between 7000 and 9000 ml was processed in each apheresis. Under these conditions, a total of 64 apheresis procedures was performed in the 20 patients. The mean numbers of mononuclear cells and of CFU-GM harvested per apheresis were 4.1 x 10(8)/kg and 110 x 10(4)/kg, respectively. A number of CFU-GM sufficient for engraftment (> 30 x 10(4)/kg) could be harvested by a single apheresis in 15 of the 20 patients. So far, 11 patients have been transplanted with PBSC and obtained rapid hematopoietic recovery. The median time to recover neutrophils more than 0.5 x 10(9)/l was 10 days, and that for platelets 50 x 10(9)/l was 11 days. This method for harvesting large numbers of PBSC allows safer autotransplantation in patients with chemoradiosensitive tumors, and is applicable to older patients.