Mal de Rio Cuarto virus infection causes hormone imbalance and sugar accumulation in wheat leaves
Mal de Rio Cuarto virus infection causes hormone imbalance and sugar accumulation in wheat leaves
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DOI：
10.1186/s12870-019-1709-y
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发表时间：
2019-03-22
影响因子：
5.3
通讯作者：
del Vas, Mariana
中科院分区：
文献类型：
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作者：
Alejandro de Haro, Luis;Maite Arellano, Sofia;del Vas, Mariana
背景：小麦冠状病毒（MRCV）感染多种单子叶植物，包括玉米和小麦。感染植株节间缩短，部分不育，分蘖增加，根长减少。为了更好地了解导致这些症状的植物与病毒相互作用的分子基础，我们将RNA测序与代谢物和激素测量相结合。结果：在mrcv感染的小麦植株中，与模拟接种植株相比，接种21天后检测到3000多个差异积累转录本（dat）。受感染的植株表现出与蔗糖韧皮部负荷有关的TaSWEET13转录本水平下降。可溶性糖、淀粉、海藻糖6-磷酸（Tre6P）、有机氨基酸和氨基酸在mrcv感染植株中含量均较高。此外，一些与植物激素代谢、运输和信号传导相关的转录本在MRCV感染后增加。编码GA20ox、D14、MAX2和smax1样蛋白的转录本减少，这些蛋白参与赤霉素生物合成和独角麦内酯信号传导。参与茉莉酸、乙烯和油菜素类固醇生物合成、感知和信号传导以及生长素运输的转录本也发生了改变。激素测量表明，茉莉酸、油菜素内酯、脱落酸和吲哚-3-乙酸在侵染叶片中显著升高。结论：我们的研究结果表明，MRCV引起严重的激素失衡，加上糖分配的改变，可以解释MRCV感染植物中观察到的症状。
Background: Mal de Rio Cuarto virus (MRCV) infects several monocotyledonous species including maize and wheat. Infected plants show shortened internodes, partial sterility, increased tillering and reduced root length. To better understand the molecular basis of the plant-virus interactions leading to these symptoms, we combined RNA sequencing with metabolite and hormone measurements.Results: More than 3000 differentially accumulated transcripts (DATs) were detected in MRCV-infected wheat plants at 21 days post inoculation compared to mock-inoculated plants. Infected plants exhibited decreased levels of TaSWEET13 transcripts, which are involved in sucrose phloem loading. Soluble sugars, starch, trehalose 6-phosphate (Tre6P), and organic and amino acids were all higher in MRCV-infected plants. In addition, several transcripts related to plant hormone metabolism, transport and signalling were increased upon MRCV infection. Transcripts coding for GA20ox, D14, MAX2 and SMAX1-like proteins involved in gibberellin biosynthesis and strigolactone signalling, were reduced. Transcripts involved in jasmonic acid, ethylene and brassinosteroid biosynthesis, perception and signalling and in auxin transport were also altered. Hormone measurements showed that jasmonic acid, brassinosteroids, abscisic acid and indole-3-acetic acid were significantly higher in infected leaves.Conclusions: Our results indicate that MRCV causes a profound hormonal imbalance that, together with alterations in sugar partitioning, could account for the symptoms observed in MRCV-infected plants.