Haemato-biochemical disorders associated with nodavirus like-agent in adult leaping mullet Liza saliens (Risso, 1810) in the Caspian Sea
Haemato-biochemical disorders associated with nodavirus like-agent in adult leaping mullet Liza saliens (Risso, 1810) in the Caspian Sea
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DOI：
10.1007/s13337-015-0289-1
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发表时间：
2016-03-01
期刊：
影响因子：
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通讯作者：
Zorriehzahra, Mohamad Jalil
中科院分区：
文献类型：
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作者：
Ghiasi, Maryam;Binaii, Mohammad;Zorriehzahra, Mohamad Jalil
Betanoda病毒是世界各地不同地理区域的几种海洋鱼类中的一个新问题。近年来，里海的乌鱼数量急剧下降，并引入了一种甜菜碱病毒样因子作为乌鱼死亡的原因。本研究的主要目的是比较血液生化参数在健康和感染betanoda病毒样剂的尖吻乌鱼（Liza saliens）。成年尖鼻乌鱼（34个临床受影响的鱼？从2012年到2013年，从里海东南部捕获了34条明显健康的鱼。所有捕获的鱼都是4或5岁。感染鱼的主要临床症状为嗜睡、严重腹胀、游泳异常和鱼鳔过度膨胀。结果表明，感染鱼的体重和全长均显著低于健康鱼。组织病理学检查和间接荧光抗体试验结果证实了感染鱼体内存在甜菜碱病毒样因子。病鱼严重贫血（低色素性大红细胞贫血），并有严重的造血功能障碍。贫血伴有严重的白细胞减少，中性粒细胞和幼稚中性粒细胞显著升高，淋巴细胞百分比显著下降。总蛋白，白蛋白和总免疫球蛋白水平显着降低，而天冬氨酸氨基转移酶和丙氨酸氨基转移酶的活性显着增加时相比，健康的鱼。这些结果表明，在里海伊朗水域，感染甜菜碱病毒样因子的鱼的摄食受到干扰，可能导致鱼的血液生化紊乱和死亡。
Betanoda virus is an emerging problem in several marine fish species in various geographic areas all over the world. In recent years, mullets stock of the Caspian Sea decreased dramatically and a betanoda virus like-agent was introduced as the cause of mullet's mortality. The main objective of the present study is to compare hemato-biochemical parameters in healthy and infected sharpnose mullets (Liza saliens) to betanoda virus like-agent. The adult sharpnose mullets (34 clinically affected fish ? 34 apparently healthy fish) were captured from the southeast the Caspian Sea from 2012 to 2013. All of the captured fish were 4 or 5 years old. The main clinical sings of infected fish were lethargy, severe abdominal distention, abnormal swimming and hyperinflation of swim bladder. The results showed that the weight and total length of infected fish were significantly lower than the healthy fish. The results of histopatological evaluation and indirect florescent antibody test were confirmed the presence of a betanoda virus-like agent in infected fish. The diseased fish were severely anemic (hypochromic macrocytic anemia) and had a serious haemopoietic disorders. The anemia associated with a sever leukopenia, a significant rise of neutrophils and immature neutrophils and a significant decries of lymphocytes percentage. Total protein, albumin and total immunoglobulin levels were significantly reduced in the serum of infected fish, while the activities of aspartate aminotransferase and alanine aminotransferase significantly increased when compared to the healthy fish. These results suggested that the feeding of the infected fish to betanoda virus like-agent were disturbed and it could be cause haemato-biochemical disorder and mortality of the fish in Iranian water of the Caspian Sea.