Characteristics of alpha smooth muscle actin-positive peritubular cells in prepubertal bovine testes
Characteristics of alpha smooth muscle actin-positive peritubular cells in prepubertal bovine testes
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DOI：
10.1016/j.bbrc.2022.03.149
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发表时间：
2022-04-09
影响因子：
3.1
通讯作者：
Hara，Kenshiro
中科院分区：
文献类型：
--
作者：
Kawabe，Yusuke;Numabe，Takashi;Hara，Kenshiro
性成熟牛的管周肌样细胞（PMC）的特征仍然很大程度上未知。在这里，我们报告了从 5 个月大的公牛获得的青春期前睾丸中表达α平滑肌肌动蛋白 (αSMA) 的管周细胞的特征和行为，αSMA 是成年睾丸中 PMC 的标志物。在青春期前的睾丸中，αSMA + PMC 周围的弹性蛋白分布暗淡且不连续，但在成年睾丸中分布强烈且连续。成纤维细胞特异性蛋白 1 (FSP1) 在青春期前睾丸的 αSMA + PMC 中很少表达，而在成年睾丸中，大多数 αSMA + PMC 为 FSP1+。此外，青春期前睾丸中的 αSMA + PMC 比成年睾丸增殖更活跃。对青春期前睾丸分离的生精小管进行体外培养，结果显示 αSMA + PMC 从管周迁移到间质区域。因此，在青春期前的牛睾丸中，(1) PMC 很少表现出成纤维细胞样特性，(2) PMC 在 FSP1 表达中表现出异质性，(3) PMC 比成年睾丸中的 PMC 增殖更活跃，(4) PMC 有迁移到间质的潜力。我们的观察有助于了解 PMC 的成熟及其对牛睾丸功能的参与。
Characteristics of peritubular myoid cells (PMCs) in sexually immature cattle remain largely unknown. Here, we report the character and behavior of peritubular cells expressing alpha smooth muscle actin (αSMA), a marker of PMCs in adult testes, in prepubertal testes procured from 5-months-old bulls. Elastin distribution around αSMA + PMCs was dim and discontinuous in prepubertal testes, but strong and continuous in adult testes. Fibroblast-specific protein 1 (FSP1) was rarely expressed in αSMA + PMCs of prepubertal testes, while in adult testes, majority of αSMA + PMCs were FSP1+. Moreover, αSMA + PMCs in prepubertal testes proliferated more actively than those in adult testes.In vitroculture of isolated seminiferous tubules from prepubertal testes revealed that αSMA + PMCs migrate from peritubular to interstitial area. Hence, in prepubertal bovine testes, (1) PMCs rarely portray fibroblast-like properties, (2) PMCs exhibit heterogeneity in FSP1 expression, (3) PMCs proliferate more actively than those in adult testes, and (4) PMCs have a potential to migrate to the interstitium. Our observations help to understand the maturation of PMCs and their involvement in bovine testicular function.