RISC activity in hippocampus is essential for contextual memory
RISC activity in hippocampus is essential for contextual memory
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DOI：
10.1016/j.neulet.2010.01.038
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发表时间：
2010-03-08
影响因子：
2.5
通讯作者：
Cestari, Vincenzo
中科院分区：
文献类型：
--
作者：
Batassa, Enrico Maria;Costanzi, Marco;Cestari, Vincenzo
RNA诱导沉默复合物（RISC）介导基因表达的转录后控制，并含有Argonaute 2（AGO 2）蛋白作为mRNA翻译的切割或抑制的中心效应物。在大脑中，RISC通路参与神经元功能，如突触发育和局部蛋白质合成，这对记忆可能至关重要。在这项研究中，我们研究了RISC在啮齿动物记忆形成中的作用，通过沉默AGO 2在C57 BL/6小鼠背海马的表达，并提交动物的海马相关的任务。手术后一周，AGO 2下调损害了短期和长期的背景恐惧记忆。相反，在手术后三周，当AGO 2水平重新建立时，没有观察到持久的影响。这些结果表明，RISC活动的改变严重影响啮齿动物的学习和记忆过程。(C)2010爱思唯尔爱尔兰有限公司版权所有。
RNA-Induced Silencing Complex (RISC) mediates post-transcriptional Control of gene expression and contains Argonaute 2 (AGO2) protein as a central effector of cleavage OF inhibition of mRNA translation. In the brain, the RISC pathway is involved in neuronal functions, Such as synaptic development and local protein synthesis, which are potentially critical for memory. In this study, we examined the role of RISC in memory formation in rodents, by silencing AGO2 expression in dorsal hippocampus of C57BL/6 mice and submitting animals to hippocampus-related tasks. One week after surgery, AGO2 downregulation impaired both short-term and long-term contextual fear memories. Conversely, no long-lasting effects were observed three weeks after surgery, when AGO2 levels were re-established. These results show that altered RISC activity severely affects learning and memory processes in rodents. (C) 2010 Elsevier Ireland Ltd. All rights reserved.