Overexpression of Brassica napus cytosolic fructose-1,6-bisphosphatase and sedoheptulose-1,7-bisphosphatase genes significantly enhanced tobacco growth and biomass
Overexpression of Brassica napus cytosolic fructose-1,6-bisphosphatase and sedoheptulose-1,7-bisphosphatase genes significantly enhanced tobacco growth and biomass
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DOI：
10.1016/s2095-3119(20)63438-4
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发表时间：
2022-01-04
影响因子：
4.8
通讯作者：
Zhang Rui
中科院分区：
文献类型：
--
作者：
Li Yan-yan;Guo Li-na;Zhang Rui
胞质果糖 1,6-二磷酸酶 (cyFBPase) 和景天庚酮糖 1,7-二磷酸酶 (SBPase) 活性的升高与植物的高产量相关。在这项研究中，通过在烟草 (Nicotiana tabacum) 植物中过表达油菜 (Brassica napus) cDNA 来提高 cyFBPase 和 SBPase 基因的表达水平。与野生型（WT）相比，共表达cyFBPase和SBPase（TpFS）或表达单一cyFBPase（TpF）或SBPase（TpS）的转基因植物的cyFBPase活性分别是野生型（WT）的1.77倍、1.55倍、1.23倍和SBPase活性1.45倍、1.12倍、1.36倍。与WT植物相比，TpF、TpS和TpFS的光合作用率分别提高了4%、20%和25%。 SBPase和cyFBPase在转基因烟草植物中相互正向调节并协同发挥作用。此外，三种转基因植物的蔗糖含量均高于WT植物。 TpFS和TpS植物的淀粉积累分别提高了53%和37%，但TpF植物的淀粉积累略有下降。此外，携带SBPase和/或cyFBPase基因的转基因烟草植物显示出其生长、生物量、干重、株高、茎直径、叶大小、花数和荚重量的改善。总之，SBPase和cyFBPase的共表达可能为农业应用中提高作物产量开辟新途径。
Elevated activities of cytosolic fructose-1,6-bisphosphatase (cyFBPase) and sedoheptulose-1,7-bisphosphatase (SBPase) are associated with higher yields in plants. In this study, the expression levels of the cyFBPase and SBPase genes were increased by overexpressing rape (Brassica napus) cDNA in tobacco (Nicotiana tabacum) plants. The transgenic plants coexpressing cyFBPase and SBPase (TpFS), or expressing single cyFBPase (TpF) or SBPase (TpS) had 1.77-, 1.55-, 1.23-fold cyFBPase and 1.45-, 1.12-, 1.36-fold SBPase activities as compared to the wild-type (WT), respectively. Photosynthesis rates of TpF, TpS and TpFS increased 4, 20 and 25% compared with WT plants. The SBPase and cyFBPase positively regulated each other and functioned synergistically in transgenic tobacco plants. In addition, the sucrose contents of the three transgenic plants were higher than that of WT plants. The starch accumulation of the TpFS and TpS plants was improved by 53 and 37%, but slightly decreased in TpF plants. Moreover, the transgenic tobacco plants harbouring SBPase and/or cyFBPase genes showed improvements in their growth, biomass, dry weight, plant height, stem diameter, leaf size, flower number, and pod weight. In conclusion, co-expression of SBPase and cyFBPase may pave a new way for improving crop yield in agricultural applications.