CHANGES IN LH, PROGESTERONE AND SEXUAL-BEHAVIOR ASSOCIATED WITH PREOVULATORY LUTEINIZATION IN BITCH
CHANGES IN LH, PROGESTERONE AND SEXUAL-BEHAVIOR ASSOCIATED WITH PREOVULATORY LUTEINIZATION IN BITCH
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DOI：
10.1095/biolreprod17.4.604
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发表时间：
1977-01-01
影响因子：
3.6
通讯作者：
MCENTEE, K
中科院分区：
文献类型：
--
作者：
CONCANNON, P;HANSEL, W;MCENTEE, K
在发情前期和发情期，每天0800，1600和2400 h从6只比格犬中采集血浆，测定LH [促黄体生成激素]和孕酮。每天评估母犬对雄性的性行为反应。排卵前LH峰持续24-40 h，在发情开始前3天和发情开始后1天之间的2400或0800 h达到峰值（4.4-11.1 ng/ml）。每只母犬的发情行为仅在孕酮增加后观察到。孕酮的初始增加发生在LH峰开始之前或同时。平均值（±）在LH峰后-56、-40、-24、-16、-8、0、24和48 h的孕酮SEM [平均值的标准误差]为0.7 ±。0.1，0.9 .+-。0.1、1.2 .+-. 0.1，1.4 . ±. 0.2，1.5 . ±. 0.2，2.6 . ±. 0.3，3.2 . ±. 0.4和5.4 .+-。0.6 ng/ml。同时LH为1.7 ±。0.3，1.1 . ±. 0.2，1.5 . ±. 0.3，1.8 . ±. 0.3，4.9 . ±. 1.0，7.3 .+-。1.0，2.0 .+-. 0.3和1.4 .+-。0.4 ng/ml。通过将发情前期或发情期间从10只比格犬中取出的卵巢的大体和组织学检查结果与在获得卵巢前每天采集2-3次的血浆中测量的LH和孕酮相关联，估计母犬的排卵时间。在LH峰值后约4、8、20、38、44和50 h，当血浆孕酮分别为1.3、2.8、4.2、3.3、4.4和6.9 ng/ml时，从其中6只母犬中取出卵巢。排卵与未排卵的成熟卵泡比例分别为0：10、0：6、0：7、0：8、4：3和11：0。每天观察6只去卵巢母犬的性行为，连续5周，在此期间，在开始16天的孕酮治疗前8天或后8天开始16天的雌激素治疗。初始孕酮（4.8 ±. 0.4 ng/ml血浆）没有影响。在叠加雌激素（65 . ±. 5 μ g/ml血浆）。最初的雌激素导致母犬对雄性有吸引力，但未能诱导雄性接受。完全的性感受性，诱导内18小时内叠加孕激素对雌激素，不受影响的去除雌激素8天后，但完全失去了孕激素去除后的18小时内，另一个8天。母犬的排卵明显发生在发情开始前1天至发情开始后5天之间，大约在LH峰值后38-44小时。卵泡黄体化和孕酮分泌增加在排卵前60-70小时LH峰开始之前或同时开始。排卵前孕激素的初始升高与发情前晚期雌激素峰值在诱导发情行为中是协同作用的。
LH [luteinizing hormone] and progesterone were assayed in plasma collected at 0800, 1600 and 2400 h daily from each of 6 beagles during proestrus and estrus. The bitches'' sexual behavior in response to males was assessed daily. Preovulatory LH surges lasting 24-40 h peaked (4.4-11.1 ng/ml) at 2400 or 0800 h between 3 days before and 1 day after the onset of estrus. Estrous behavior in each bitch was observed only after an increase in progesterone. The initial increase in progesterone occurred either just prior to, or concomitant with, the initiation of the LH surge. Mean (.+-. SEM [standard error of the mean]) progesterone at -56, -40, -24, -16, -8, 0, 24 and 48 h from the LH peak was 0.7 .+-. 0.1, 0.9 .+-. 0.1, 1.2 .+-. 0.1, 1.4 .+-. 0.2, 1.5 .+-. 0.2, 2.6 .+-. 0.3, 3.2 .+-. 0.4 and 5.4 .+-. 0.6 ng/ml. LH at the same times was 1.7 .+-. 0.3, 1.1 .+-. 0.2, 1.5 .+-. 0.3, 1.8 .+-. 0.3, 4.9 .+-. 1.0, 7.3 .+-. 1.0, 2.0 .+-. 0.3 and 1.4 .+-. 0.4 ng/ml. Ovulation time in the bitch was estimated by correlating results of gross and histological examination of ovaries removed from each of 10 beagles durin proestrus or estrus with LH and progesterone measured in plasma collected 2-3 times a day prior to obtaining the ovaries. Ovaries were removed from 6 of these bitches at approximately 4, 8, 20, 38, 44 and 50 h after the LH peak, when plasma progesterone was 1.3, 2.8, 4.2, 3.3, 4.4 and 6.9 ng/ml, respectively. The respective ovulated:nonovulated mature follicle ratios were 0:10, 0:6, 0:7, 0:8, 4:3 and 11:0. The sexual behavior of 6 ovariectomized bitches was observed daily for 5 wk during which 16 days of estrogen treatment was initiated either 8 days before or 8 days after initiation of a 16 day progesterone treatment. Initial progesterone (4.8 .+-. 0.4 ng/ml plasma) had no effect. Induction of full sexual receptivity occurred 8 days after superimposing estrogen (65 .+-. 5 pg/ml plasma) on progesterone. Initial estrogen caused bitches to be attractive to males but failed to induce any acceptance of the males. Full sexual receptivity was induced within 18 h of superimposing progesterone on estrogen, was not affected by removal of estrogen 8 days later but was lost entirely within 18 h of progesterone removal after another 8 days. In the bitch ovulation apparently occurs between 1 day before and 5 days after the onset of estrus and approximately 38-44 h following the LH peak. Follicle luteinization and increased progesterone secretion begins just prior to or concomitant with initiation of the LH surge some 60-70 h prior to ovulation. The initial preovulatory rise in progesterone is synergistic with the preceding late-proestrous estrogen peak in eliciting estrous behavior.