Acceleration of hard and soft tissue healing in the oral cavity by a single transmucosal injection of fluvastatin-impregnated poly (lactic-co-glycolic acid) microspheres. An in vitro and rodent in vivo study
Acceleration of hard and soft tissue healing in the oral cavity by a single transmucosal injection of fluvastatin-impregnated poly (lactic-co-glycolic acid) microspheres. An in vitro and rodent in vivo study
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DOI：
10.1088/1748-6041/11/1/015001
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发表时间：
2015-12
影响因子：
4
通讯作者：
Noriyuki Yasunami;Y. Ayukawa;A. Furuhashi;I. Atsuta;Y. D. Rakhmatia;Yasuko Moriyama;T. Masuzaki;K. K
中科院分区：
文献类型：
--
作者：
Noriyuki Yasunami;Y. Ayukawa;A. Furuhashi;I. Atsuta;Y. D. Rakhmatia;Yasuko Moriyama;T. Masuzaki;K. K
据报道，抗高血压药物他汀类药物促进骨形成和软组织愈合。我们研究了缓释氟伐他汀浸渍的聚乳酸-羟基乙酸共聚物（PLGA）微球在体内促进拔牙窝骨和牙龈愈合的效果，以及氟伐他汀在体外对上皮细胞和成纤维细胞的影响。拔除大鼠上颌右侧第一磨牙，然后立即将以下药物之一单次注射到牙龈颊襞：对照组（未给药）、不含他汀类药物的PLGA微球（活性对照组）或含20或40 μg kg−1氟伐他汀的PLGA微球。在注射后第1、3、7、14和28天，对骨和软组织愈合进行组织学评价。在0、0.01、0.1、1.0、10和50 μM剂量的氟伐他汀作用下测量细胞增殖。在0和0.1 μM剂量下观察细胞迁移和形态。拔牙后，他汀类药物显着增加骨体积和密度，结缔组织体积，上皮伤口愈合。在体外研究中，它促进上皮细胞和成纤维细胞的显著增殖和迁移。单剂量局部给予氟伐他汀浸渍的PLGA微球促进拔牙部位的骨和软组织愈合。
Antihyperlipidemic drug statins reportedly promote both bone formation and soft tissue healing. We examined the effect of sustained-release, fluvastatin-impregnated poly(lactic-co-glycolic acid) (PLGA) microspheres on the promotion of bone and gingival healing at an extraction socket in vivo, and the effect of fluvastatin on epithelial cells and fibroblasts in vitro. The maxillary right first molar was extracted in rats, then one of the following was immediately injected, as a single dose, into the gingivobuccal fold: control (no administration), PLGA microspheres without a statin (active control), or PLGA microspheres containing 20 or 40 μg kg−1 of fluvastatin. At days 1, 3, 7, 14, and 28 after injection, bone and soft tissue healing were histologically evaluated. Cell proliferation was measured under the effect of fluvastatin at dosages of 0, 0.01, 0.1, 1.0, 10, and 50 μM. Cell migration and morphology were observed at dosages of 0 and 0.1 μM. Following tooth extraction, the statin significantly enhanced bone volume and density, connective tissue volume, and epithelial wound healing. In the in vitro study, it promoted significant proliferation and migration of epithelial cells and fibroblasts. A single dose of topically administered fluvastatin-impregnated PLGA microspheres promoted bone and soft tissue healing at the extraction site.