Complete genome sequence data of multidrug-resistant Stenotrophomonas sp. strain SXG-1.
Complete genome sequence data of multidrug-resistant Stenotrophomonas sp. strain SXG-1.
复制标题
多重耐药寡养单胞菌的完整基因组序列数据。
DOI：
10.1016/j.jgar.2020.03.005
复制
发表时间：
2020
影响因子：
4.6
通讯作者：
S. Tao
中科院分区：
文献类型：
--
作者：
Xiaoping Zhang;Baodi Shang;Xiaoyi Li;Zhengyou Li;S. Tao
目的研究一株多重耐药的寡养单胞菌。方法采用Illumina HiSeq 2500-PE 125平台，利用MPS（massively parallel sequencing）技术，对SXG-1进行全基因组测序。使用SOAPdenovo将所有良好质量的配对读段组装到许多支架中。结果报道了寡养单胞菌SXG-1的全基因组序列，包含4077个编码序列（CDS），全长4534，602 bp，G + C含量为66.42%。基因组包含4个基因岛，72个tRNA和13个rRNA。注释分析表明，SXG-1株编码22个与多药耐药相关的基因。除10个通过不同机制产生耐药性的基因外，还发现了12个外排泵基因，其中9个为嗜麦芽寡养单胞菌首次报道。从鲟鱼中分离出来Stenotrophomonassp. SXG-1菌株的耐药性可能有助于了解细菌的耐药机制和预防疾病。
Objectives A multidrug-resistant bacterium,Stenotrophomonassp. SXG-1, was isolated from the liver of diseased hybrid sturgeon from Guizhou province, China.Methods Whole-genome sequencing was performed on the Illumina HiSeq 2500-PE125 platform with MPS (massively parallel sequencing) Illumina technology. All good quality paired reads were assembled using the SOAPdenovo into a number of scaffolds. PHI (Pathogen Host Interactions), VFDB (Virulence Factors of Pathogenic Bacteria) and ARDB (Antibiotic Resistance Genes Database) were used to analyses pathogenicity and drug resistance.Results Here we reported the complete genome sequence ofStenotrophomonas sp.SXG-1, which comprised 4534,602 bp in 4077 coding sequences (CDS) with a G + C content of 66.42%. The genome contained 4 gene islands, 72 tRNAs and 13 rRNAs. According to the annotation analysis, strain SXG-1 encoded 22 genes related to the multidrug resistance. In addition to 10 genes conferring resistance to antimicrobial drugs of different classes via alternative mechanisms, 12 genes of efflux pumps were presented, 9 of which were reported for the first time inStenotrophomonas maltophilia.Conclusion This was the first complete genome sequence ofStenotrophomonassp. isolated from the sturgeon. The complete genome sequence ofStenotrophomonassp. strain SXG-1 may provide insights into the mechanism of antimicrobial resistance and prevent disease.