MAIZE REGULATORY GENE OPAQUE-2 ENCODES A PROTEIN WITH A LEUCINE-ZIPPER MOTIF THAT BINDS TO ZEIN DNA
MAIZE REGULATORY GENE OPAQUE-2 ENCODES A PROTEIN WITH A LEUCINE-ZIPPER MOTIF THAT BINDS TO ZEIN DNA
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DOI：
10.1073/pnas.87.1.46
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发表时间：
1990-01-01
影响因子：
11.1
通讯作者：
BURR, B
中科院分区：
文献类型：
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作者：
SCHMIDT, RJ;BURR, FA;BURR, B
玉米中的opaque-2基因座（o2）调节玉米醇溶蛋白多基因家族贮藏蛋白的许多成员的表达。从玉米胚乳cDNA文库中分离（O2）基因座（O2）的野生型等位基因的cDNA克隆并测序。我们发现了一个258个核苷酸的5“前导序列，它含有三个短的开放阅读框，后面是一个437个氨基酸的蛋白质序列。由O2 cDNA指定的蛋白质（O2）的假定氨基酸序列含有一些哺乳动物和真菌转录激活因子的特征性“亮氨酸拉链”结构域。lacZ-O2融合结构，使用几乎整个编码区的O2或仅指定的亮氨酸拉链结构域的片段，在大肠杆菌中表达。在体外结合测定中，β-半乳糖苷酶-O2融合蛋白结合到玉米醇溶蛋白基因组克隆中编码序列5“侧的两个特定区域。这表明O2蛋白通过与一个或多个玉米醇溶蛋白启动子元件直接相互作用来影响玉米醇溶蛋白的转录。
The opaque-2 locus (o2) in maize regulates the expression of many members of the zein multigene family of storage proteins. cDNA clones for a wild-type allele of the (o2) locus (O2) were isolated from a maize endosperm cDNA library and sequenced. We found a 258-nucleotide 5'' leader sequence containing three short open reading frames follwed by a sequence specifying a protein of 437 amino acids. The presumptive amino acid sequence of the protein (O2) specified by the O2 cDNA contains a "leucine-zipper" domain characteristic of some mammalian and fungal transcription activation factors. lacZ-O2 fusion constructs, using nearly the entire coding region of O2 or only a fragment specifying the leucine-zipper domain, were expressed in Escherichia coli. In an in vitro binding assay, the .beta.-galactosidase-O2 fusion proteins bound to two specific regions on the 5'' side of the coding sequence in a zein genomic clone. This suggests that the O2 protein affects zein transcription through direct interaction with one or more zein promoter elements.