FUNCTIONAL-ANALYSIS OF BACTERIOPHAGE-F1 INTERGENIC REGION
FUNCTIONAL-ANALYSIS OF BACTERIOPHAGE-F1 INTERGENIC REGION
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DOI：
10.1016/0042-6822(81)90226-9
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发表时间：
1981-01-01
期刊：
影响因子：
3.7
通讯作者：
ZINDER, ND
中科院分区：
文献类型：
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作者：
DOTTO, GP;ENEA, V;ZINDER, ND
噬菌体F1基因组包含一个基因间隔区(IG)，长度为508个核苷酸，不编码任何已知蛋白质。利用含有该区域不同部分和F1基因组相邻部分的嵌合质粒，确定了3个功能结构域：基因II的近端负责阻止F1和F2的吸附和穿透(IP2表型)；IG的中间部分负责F1复制的功能起源；以及基因IV/IG连接处的一个序列对颗粒形态发生重要。这些区域可以插入到一个质粒的不同位置，并且彼此独立地发挥作用。
The bacteriophage f1 genome contains an intergenic region (IG) 508 nucleotides long that does not code for any known protein. By using chimeric plasmids harboring different portions of this region and neighboring parts of the f1 genome, 3 functional domains were identified: the proximal part of gene II, responsible for the prevention of f1 and f2 adsorption and penetration (IP2 phenotype); a part in the middle of the IG responsible for the f1 functional origin of replication; and a sequence at the gene IV/IG junction important for particle morphogenesis. These regions can be inserted into separate sites of a plasmid and function independently of one another.