Polycationic protamine for water-insoluble complex formation with DNA
Polycationic protamine for water-insoluble complex formation with DNA
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DOI：
10.4012/dmj.2009-131
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发表时间：
2010-09-01
影响因子：
2.5
通讯作者：
Mitarai, Makoto
中科院分区：
文献类型：
--
作者：
Fukushima, Tadao;Ohno, Jun;Mitarai, Makoto
将硫酸鱼精蛋白溶液与DNA在搅拌下反应，制备了DNA/鱼精蛋白复合物，并对其细胞活力、抑菌效果和组织病理学反应进行了检测。得到具有多孔结构的水不溶性白色粉末，DNA/鱼精蛋白复合物。由含有等量DNA和硫酸鱼精蛋白的溶液制备的复合物的摩尔结合比为0.038，复合物形成效率为61%。在使用MC-3 T3-E1小鼠成骨细胞的细胞培养试验中，复合物显示出比单独的硫酸鱼精蛋白更小的细胞毒性，并且细胞存活率大于98%。多孔盘可以容易地制备，并且在抗菌敏感性测试和体内测试中显示出对金黄色葡萄球菌、牙龈卟啉单胞菌和中间普氏菌的抗菌效果。这些结果表明，DNA/鱼精蛋白复合物可能是一种有用的生物降解材料，具有抗菌作用。
The DNA/protamine complex was prepared by a reaction between DNA and protamine sulfate solutions with stirring, and its cell viability, antibacterial effect and histopathological responses were examined. A water-insoluble white powder, DNA/protamine complex, with a porous structure was obtained. The molar binding ratio of the complex prepared from a solution containing equal amounts of DNA and protamine sulfate by weight was 0.038 and the efficiency of complex formation was 61%. In a cell culture test using MC-3T3-E1 mouse osteoblast cells, the complex showed less cytotoxicity than protamine sulfate alone and cell viabilities were more than 98%. A porous disk could be prepared easily and showed an antibacterial effect against Staphyrococcus aureus, Porphyromonas gingivalis and Prevotella intermedia in an antibacterial sensitivity test and a mild tissue response in vivo test. These results suggested that the DNA/protamine complex could be a useful biodegradable biomaterial with antibacterial effects.