COMBINED INTERFERON-ALPHA-2, RIMANTADINE HYDROCHLORIDE, AND RIBAVIRIN INHIBITION OF INFLUENZA-VIRUS REPLICATION INVITRO
COMBINED INTERFERON-ALPHA-2, RIMANTADINE HYDROCHLORIDE, AND RIBAVIRIN INHIBITION OF INFLUENZA-VIRUS REPLICATION INVITRO
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DOI：
10.1128/aac.25.1.53
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发表时间：
1984-01-01
影响因子：
4.9
通讯作者：
PUSHKARSKAYA, NL
中科院分区：
文献类型：
--
作者：
HAYDEN, FG;SCHLEPUSHKIN, AN;PUSHKARSKAYA, NL
重组dna产生人干扰素。2抑制甲型和乙型流感病毒在原代恒河猴肾细胞（RMK）中的复制。人类的干扰素-.alpha。2 .与盐酸金刚乙胺或利巴韦林相互作用或协同作用，降低临床分离的H3N2或H1N1亚型甲型流感病毒的产量。人类干扰素。2和利巴韦林对乙型流感病毒复制的抑制程度也比任何一种单一药物都要大。除药物浓度外，病毒接种量和培养时间是决定抑制程度的重要变量。单药或联合用药并未显著抑制未感染RMK细胞的生长，这表明观察到的抗病毒活性相互作用不是由于细胞毒性。
Recombinant DNA-produced human interferon-.alpha.2 inhibited the replication of influenza A and B viruses in primary rhesus monkey kidney cells (RMK). Human interferon-.alpha.2 interacted additively or synergistically with rimantadine hydrochloride or ribavirin in reducing the yield of clinical isolates of H3N2 or H1N1 subtype influenza A viruses. The combination of human interferon-.alpha.2 and ribavirin also inhibited the replication of an influenza B virus to a greater extent than either single agent. In addition to drug concentration, the virus inoculum and duration of culture were important variables in determining the degree of inhibition. Single drugs or combinations did not significantly inhibit the growth of uninfected RMK cells, which indicated that the observed interactions with respect to antiviral activity were not due to cell cytotoxicity.