Repair Effect of Seaweed Polysaccharides with Different Contents of Sulfate Group and Molecular Weights on Damaged HK-2 Cells
Repair Effect of Seaweed Polysaccharides with Different Contents of Sulfate Group and Molecular Weights on Damaged HK-2 Cells
复制标题
不同硫酸基含量和分子量的海藻多糖对受损HK-2细胞的修复作用
DOI：
10.3390/polym8050188
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发表时间：
2016-05-01
期刊：
影响因子：
5
通讯作者：
Yu, Kai
中科院分区：
文献类型：
--
作者：
Bhadja, Poonam;Tan, Cai-Yan;Yu, Kai
研究了6种低分子量海藻多糖（SPS）对琥珀酸诱导的人近端肾小管上皮细胞（HK-2）损伤的构效关系及其修复机制。这些SPS包括海带多糖、降解条斑紫菜多糖、降解龙须菜多糖、降解羊栖菜多糖、降解麒麟菜多糖和降解裙带菜多糖。通过控制H_2O_2浓度降解后，这些SPS的分子量差异很小（从1968到4020 Da）。6种SPS的硫酸根（-SO 3 H）含量分别为21.7%、17.9%、13.3%、8.2%、7.0%和5.5%，-COOH含量在1.0%~ 1.7%之间。降解后，多糖的特征-SO 3 H和-COOH基团的含量没有显着差异。采用CCK-8法检测细胞活力，苏木精-伊红染色法检测细胞形态学，观察多糖对细胞损伤的修复作用。结果表明，0.1 ~ 100 μ g/mL的多糖对HK-2细胞无细胞毒性，对琥珀酸诱导的HK-2细胞损伤具有修复作用。同时，多糖-SO 3 H含量与修复能力呈正相关。此外，低分子量的降解多糖表现出更好的修复活性损伤的HK-2细胞比其未降解的对应物。本研究结果可为抑制肾结石的形成和开发抗结石多糖类新药提供参考。
The structure-activity relationships and repair mechanism of six low-molecular-weight seaweed polysaccharides (SPSs) on oxalate-induced damaged human kidney proximal tubular epithelial cells (HK-2) were investigated. These SPSs included Laminaria japonica polysaccharide, degraded Porphyra yezoensis polysaccharide, degraded Gracilaria lemaneiformis polysaccharide, degraded Sargassum fusiforme polysaccharide, Eucheuma gelatinae polysaccharide, and degraded Undaria pinnatifida polysaccharide. These SPSs have a narrow difference of molecular weight (from 1968 to 4020 Da) after degradation by controlling H2O2 concentration. The sulfate group (-SO3H) content of the six SPSs was 21.7%, 17.9%, 13.3%, 8.2%, 7.0%, and 5.5%, respectively, and the -COOH contents varied between 1.0% to 1.7%. After degradation, no significant difference was observed in the contents of characteristic -SO3H and -COOH groups of polysaccharides. The repair effect of polysaccharides was determined using cell-viability test by CCK-8 assay and cell-morphology test by hematoxylin-eosin staining. The results revealed that these SPSs within 0.1-100 g/mL did not express cytotoxicity in HK-2 cells, and each polysaccharide had a repair effect on oxalate-induced damaged HK-2 cells. Simultaneously, the content of polysaccharide -SO3H was positively correlated with repair ability. Furthermore, the low-molecular-weight degraded polysaccharides showed better repair activity on damaged HK-2 cells than their undegraded counterpart. Our results can provide reference for inhibiting the formation of kidney stones and for developing original anti-stone polysaccharide drugs.