Cell-Directed Integration into Three-Dimensional Lipid-Silica Nanostructured Matrices
Cell-Directed Integration into Three-Dimensional Lipid-Silica Nanostructured Matrices
复制标题
DOI：
10.1021/nn101793u
复制
发表时间：
2010-10-01
期刊：
影响因子：
17.1
通讯作者：
Brinker, C. Jeffrey
中科院分区：
文献类型：
--
作者：
Harper, Jason C.;Khirpin, Constantine Y.;Brinker, C. Jeffrey
我们报道了一种独特的方法，在这种方法中，活细胞将它们整合到3D固态纳米结构中。酵母细胞沉积在弱凝聚的脂质/二氧化硅薄膜中间相上，通过自催化二氧化硅缩合过程，活跃地重建表面，创建由脂类/二氧化硅中间相包裹的局部脂双层组成的全3D生物/纳米界面。值得注意的是，这种整合过程通过pH梯度的形成，专门选择活细胞而不是相应的凋亡细胞(那些正在经历程序性细胞死亡的细胞)，这一梯度催化了二氧化硅沉积，并在细胞和周围的二氧化硅基质之间形成了一个连贯的界面。添加的长链脂或辅助纳米组分定位在pH梯度内，允许开发出其他合成方法无法实现的复杂、活性和可访问的生物/纳米界面。总体而言，该方法首次演示了有源细胞定向集成到名义上的固态三维体系结构中。它承诺了一种新的方法，将“生物”和“纳米”整合到平台上，用于在单个细胞水平上研究和操纵细胞行为，并将生物与电子学、光子学和流体学相结合。
We report a unique approach in which living cells direct their integration into 3D solid-state nanostructures. Yeast cells deposited on a weakly condensed lipid/silica thin film mesophase actively reconstruct the surface to create a fully 3D bio/nano interface, composed of localized lipid bilayers enveloped by a lipid/silica mesophase, through a self-catalyzed silica condensation process. Remarkably, this integration process selects exclusively for living cells over the corresponding apoptotic cells (those undergoing programmed cell death), via the development of a pH gradient, which catalyzes silica deposition and the formation of a coherent interface between the cell and surrounding silica matrix. Added long-chain lipids or auxiliary nanocomponents are localized within the pH gradient, allowing the development of complex active and accessible bio/nano interfaces not achievable by other synthetic methods. Overall, this approach provides the first demonstration of active cell-directed integration into a nominally solid-state three-dimensional architecture. It promises a new means to integrate "bio" with "nano" into platforms useful to study and manipulate cellular behavior at the individual cell level and to interface living organisms with electronics, photonics, and fluidics.