An aptamer based surface plasmon resonance biosensor for the detection of ochratoxin A in wine and peanut oil.
An aptamer based surface plasmon resonance biosensor for the detection of ochratoxin A in wine and peanut oil.
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DOI：
10.1016/j.bios.2014.10.059
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发表时间：
2015-03
影响因子：
12.6
通讯作者：
Zhiling Zhu;Mengxue Feng;Limin Zuo;Zhentai Zhu;Fengwei Wang;Long Chen;Jinghua Li;Guangzhi Shan;S. Luo
中科院分区：
文献类型：
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作者：
Zhiling Zhu;Mengxue Feng;Limin Zuo;Zhentai Zhu;Fengwei Wang;Long Chen;Jinghua Li;Guangzhi Shan;S. Luo
赭曲霉毒素A （Ochratoxin A， OTA）是一种绿酚类真菌毒素，广泛存在于植物性食品中，对人体有害。本研究开发了一种表面等离子体共振（SPR）生物传感器，该传感器采用抗OTA适配体固定化传感器芯片，通过直接结合实验对赭曲霉毒素a （OTA）进行定量测量。将链霉亲和素蛋白作为交联剂固定在传感器芯片表面，通过链霉亲和素-生物素相互作用捕获生物素适配体。该传感器对OTA的检测范围为0.094 ~ 100 ng/mL（线性范围为0.094 ~ 10 ng/mL），最低检测限为0.005 ng/mL。在SPR生物传感器上，采用简单的液-液萃取法进行样品预处理，进一步检测葡萄酒和花生油中的OTA。加样中赭曲霉毒素A的加样回收率为86.9% ~ 116.5%，变异系数为0.2% ~ 6.9%。本研究建立的方法具有良好的分析性能，检出限远低于最大残留限，重现性和稳定性好。
Ochratoxin A (OTA), as a kind of chlorophenolic mycotoxin, exist widely in plant origin food and is harmful to human. Herein, a surface plasmon resonance (SPR) biosensor using an anti-OTA aptamer immobilized sensor chip was developed to measure ochratoxin A (OTA) quantificationally through a straightforward direct binding assay. The streptavidin protein as a crosslinker was immobilized onto the surface of a sensor chip and the biotin-aptamer was captured through streptavidin-biotin interaction. The biosensor exhibited a detection range from 0.094 to 100 ng/mL (linear range from 0.094 to 10 ng/mL) of OTA with a lower detection limit of 0.005 ng/mL. Detection of OTA in wine and peanut oil was further performed in the SPR biosensor using simple liquid–liquid extraction for sample pretreatments. Recoveries of ochratoxin A from spiked samples ranged from 86.9% to 116.5% and coefficients of variation (CVs) ranged from 0.2% to 6.9%. The developed methods in our studies showed good analytical performances with limits of detection much lower than the maximum residue limit, as well as a good reproducibility and stability.