Patterning of HeLa Cells on a Microfabricated Au-Coated ITO Substrate
Patterning of HeLa Cells on a Microfabricated Au-Coated ITO Substrate
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HeLa 细胞在微加工 au 涂层 ITO 基板上的图案化
DOI：
10.1021/la804297x
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发表时间：
2009-05-05
期刊：
影响因子：
3.9
通讯作者：
Tang, Jing
中科院分区：
文献类型：
--
作者：
Jin, Li H.;Yang, Bing Y.;Tang, Jing
HeLa细胞被图案化在微细加工的镀金ITO衬底上。通过电化学湿法冲压技术溶解部分Au膜，然后将基片浸入端硫醇的甲氧基聚乙二醇(mpeg-SH)溶液进行进一步修饰。金岛上的自组装单分子层(SAM)可以在衬底上形成细胞和蛋白质抗性区域，活的HeLa细胞可以附着在暴露的ITO表面。当微结构单元的大小与细胞的大小匹配时，可以获得单细胞图案。荧光实验进一步证明，活的HeLa细胞更喜欢附着在ITO底物上，而不是mpeg-SH修饰的金岛上。
HeLa cells were patterned on a microfabricated Au-coated ITO substrate. Part of the Au film was dissolved by the electrochemical wet stamping technique, and the substrate was then immersed in a thiol-terminated methoxypoly(ethylene glycol) (mPEG-SH) solution for further modification. Self-assembled monolayers (SAMs) on the gold island can form cell- and protein-resistant regions on the substrate, and living HeLa cells can adhere to the exposed ITO surface. Single cell patterns could be achieved when the size of a microstructure unit matched that of the cell. The fluorescence experiments further proved that living HeLa cells prefer to attach to the ITO substrate rather than the mPEG-SH-modified gold islands.