Effects of Fiber Orientation on the Frictional Properties and Damage of Regenerative Articular Cartilage Surfaces
Effects of Fiber Orientation on the Frictional Properties and Damage of Regenerative Articular Cartilage Surfaces
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DOI：
10.1089/ten.tea.2012.0580
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发表时间：
2013-10-01
影响因子：
4.1
通讯作者：
Dini, Daniele
中科院分区：
文献类型：
--
作者：
Accardi, Mario Alberto;McCullen, Seth D.;Dini, Daniele
关节软骨提供了一个低摩擦，耐磨表面的diarodial关节。由于过载和过度使用，已知关节软骨经历显著磨损和退化，可能导致骨关节炎（OA）。再生医学策略为治疗关节软骨缺损和潜在的局部早期OA提供了一种有前途的解决方案。这些策略依赖于材料的开发来恢复软骨的某些方面。在这项研究中，不同的纤维方向（随机和对齐）的微纤维聚（-己内酯）支架与牛软骨细胞在体外培养4周，和机械和摩擦性能进行了评价。使用无侧限压缩和拉伸试验技术对力学性能进行量化。在自然关节软骨中发生的生理压缩应变下研究了摩擦特性。将支架沿着纤维方向、垂直于纤维方向和随机取向剪切。通过白色光干涉法和扫描电子显微镜评估了剪切导致的损伤演变。正如预期的那样，纤维取向强烈影响支架的拉伸性能以及压缩模量。纤维取向并没有显着影响的平衡摩擦系数，但它是，然而，一个关键因素，在支配表面上的表面损伤的演变。垂直于纤维方向的支架剪切测试显示最高的表面损伤。我们的研究结果表明，植入关节内的支架的纤维取向可能会强烈影响其对剪切损伤的抵抗力。因此，在使用微纤维支架时，应仔细考虑支架纤维的取向。
Articular cartilage provides a low-friction, wear-resistant surface for diarthrodial joints. Due to overloading and overuse, articular cartilage is known to undergo significant wear and degeneration potentially resulting in osteoarthritis (OA). Regenerative medicine strategies offer a promising solution for the treatment of articular cartilage defects and potentially localized early OA. Such strategies rely on the development of materials to restore some aspects of cartilage. In this study, microfibrous poly(-caprolactone) scaffolds of varying fiber orientations (random and aligned) were cultured with bovine chondrocytes for 4 weeks in vitro, and the mechanical and frictional properties were evaluated. Mechanical properties were quantified using unconfined compression and tensile testing techniques. Frictional properties were investigated at physiological compressive strains occurring in native articular cartilage. Scaffolds were sheared along the fiber direction, perpendicular to the fiber direction and in random orientation. The evolution of damage as a result of shear was evaluated via white light interferometry and scanning electron microscopy. As expected, the fiber orientation strongly affected the tensile properties as well as the compressive modulus of the scaffolds. Fiber orientation did not significantly affect the equilibrium frictional coefficient, but it was, however, a key factor in dictating the evolution of surface damage on the surface. Scaffolds shear tested perpendicular to the fiber orientation displayed the highest surface damage. Our results suggest that the fiber orientation of the scaffold implanted in the joint could strongly affect its resistance to damage due to shear. Scaffold fiber orientation should thus be carefully considered when using microfibrous scaffolds.