Acute toxicity and cytotoxicity of Bacillus thuringiensis and Bacillus sphaericus strains on fish and mouse bone marrow
Acute toxicity and cytotoxicity of Bacillus thuringiensis and Bacillus sphaericus strains on fish and mouse bone marrow
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DOI：
10.1007/s10646-008-0252-7
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发表时间：
2009-01-01
期刊：
影响因子：
2.7
通讯作者：
Monnerat, Rose Gomes
中科院分区：
文献类型：
--
作者：
Grisolia, Cesar Koppe;Oliveira-Filho, Eduardo Cyrino;Monnerat, Rose Gomes
苏云金芽孢杆菌(Bt)库尔斯塔克型和伊斯雷尔型β内毒素和球形芽孢杆菌(BS)H5型晶体蛋白的杀虫特性已用于杀虫数十年。微生物毒素在生物杀虫剂以及具有联合保护的植物中的可用性增加了人们对生物安全的担忧。用Bt israelsis、Bt kurstaki和BsH5菌株对尼罗罗非鱼进行了急性毒性试验和对小鼠骨髓细胞和外周血红细胞的细胞毒性试验。试验浓度和剂量分别为10(6)和10(8)个孢子/毫升。任何菌株都不能提高小鼠的致死率和对小鼠骨髓的影响。在尼罗罗非鱼外周红细胞的坏死性细胞凋亡研究中，发现暴露于苏云金芽孢杆菌菌株后，细胞坏死率增加。在两个测试系统中，暴露于球形芽孢杆菌均未显示出细胞毒性效应。与化学对照相比，研究的菌株都没有诱导细胞凋亡。
The insecticidal properties of delta-endotoxins from Bacillus thuringiensis (Bt) serotypes kurstaki and israelensis and crystal proteins of Bacillus sphaericus (Bs) serotype H5 have been used in insect control for decades. The availability of microbial toxins in biopesticides as well as in plants with incorporated protection has been increasing the concerns about biosafety. Acute toxicity to Danio rerio and cytotoxicity on mouse bone marrow cells and peripheral erythrocytes of Oreochromis niloticus were tested with Bt israelensis, Bt kurstaki and Bs H5 strains. The concentration and dose tested were 10(6) and 10(8) spores/ml, respectively. Neither lethality nor effects on mouse bone marrow were promoted by any strain. In necrosis apoptosis study on peripheral erythrocytes of O. niloticus an increased frequency of necrotic cells caused by exposure to strains of B. thuringiensis was found. Exposure to B. sphaericus did not show cytotoxic effects in either tested system. None of the strains studied induced apoptosis in contrast with the chemical controls.