Complete genomic nucleotide sequence of the temperate bacteriophage Aaφ23 of Actinobacillus actinomycetemcomitans
Complete genomic nucleotide sequence of the temperate bacteriophage Aaφ23 of Actinobacillus actinomycetemcomitans
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DOI：
10.1128/jb.186.16.5523-5528.2004
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发表时间：
2004-08-01
影响因子：
3.2
通讯作者：
Meyer, E
中科院分区：
文献类型：
--
作者：
Resch, G;Kulik, EM;Meyer, E
对伴生放线杆菌噬菌体AaPhi23的整个双链DNA基因组进行了测序。噬菌体颗粒中包含的线状DNA是环形排列的，末端是冗余的。因此，噬菌体基因组的物理图谱是圆形的。其大小为43,033个碱基，总的摩尔G+C含量为42.5摩尔%。确定了66个潜在的开放阅读框架(ORF)，其中包括一个由翻译框架漂移引起的ORF。可以将推定的功能分配给其中的23个。另外23个ORF只与几个细菌或噬菌体中存在的假设蛋白质有同源性，20个ORF似乎是噬菌体AaPhi23所特有的。噬菌体基因组的组织和一些遗传功能与Lambdoid噬菌体有广泛的相似之处。然而，AaPhi23编码一种DNA腺嘌呤甲基酶，DNA包装策略与P22系统更密切相关。AaPhi23(AttP)和几个放线菌伴生放线菌宿主(AttB)的附着位点长49个碱基。
The entire double-stranded DNA genome of the Actinobacillus actinomycetemcomitans bacteriophage AaPhi23 was sequenced. Linear DNA contained in the phage particles is circularly permuted and terminally redundant. Therefore, the physical map of the phage genome is circular. Its size is 43,033 bp with an overall molar G+C content of 42.5 mol%. Sixty-six potential open reading frames (ORFs) were identified, including an ORF resulting from a translational frameshift. A putative function could be assigned to 23 of them. Twenty-three other ORFs share homologies only with hypothetical proteins present in several bacteria or bacteriophages, and 20 ORFs seem to be specific for phage AaPhi23. The organization of the phage genome and several genetic functions share extensive similarities to that of the lambdoid phages. However, AaPhi23 encodes a DNA adenine methylase, and the DNA packaging strategy is more closely related to the P22 system. The attachment sites of AaPhi23 (attP) and several A. actinomycetemcomitans hosts (attB) are 49 bp long.