TRICARBOXYLIC ACID-CYCLE METABOLISM IN BRAIN - EFFECT OF FLUOROACETATE AND FLUOROCITRATE ON LABELLING OF GLUTAMATE, ASPARTATE, GLUTAMINE AND GAMMA-AMINOBUTYRATE
TRICARBOXYLIC ACID-CYCLE METABOLISM IN BRAIN - EFFECT OF FLUOROACETATE AND FLUOROCITRATE ON LABELLING OF GLUTAMATE, ASPARTATE, GLUTAMINE AND GAMMA-AMINOBUTYRATE
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DOI：
10.1042/bj1200345
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发表时间：
1970-01-01
影响因子：
4.1
通讯作者：
BERL, S
中科院分区：
文献类型：
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作者：
CLARKE, DD;NICKLAS, WJ;BERL, S
1.以L-[U-14C]谷氨酸、L-[U-14C]天冬氨酸、[1-14C]乙酸酯和γ-氨基[1-14C]丁酸盐为前体，研究了氟乙酸酯和柠檬酸氟对谷氨酸-谷氨酰胺系统区隔的影响。以[1-14C]乙酸酯为前体，在活体小鼠脑内研究了氟乙酸酯的作用。2.氟乙酸酯和柠檬酸氟抑制所有前体对谷氨酰胺的标记，但对谷氨酸的标记的影响要小得多。这种作用不是由于谷氨酰胺合成酶的抑制。它被解释为由于选择性地抑制了一小部分谷氨酸池的代谢，而谷氨酸池优先标记谷氨酰胺。
1. The effect of fluoroacetate and fluorocitrate on the compartmentation of the glutamate–glutamine system was studied in brain slices withl-[U-14C]glutamate,l-[U-14C]aspartate, [1-14C]acetate and γ-amino[1-14C]butyrate as precursors and in homogenates of brain tissue with [1-14C]acetate. The effect of fluoroacetate was also studiedin vivoin mouse brain with [1-14C]acetate as precursor. 2. Fluoroacetate and fluorocitrate inhibit the labelling of glutamine from all precursors but affect the labelling of glutamate to a much lesser extent. This effect is not due to inhibition of glutamine synthetase. It is interpreted as being due to selective inhibition of the metabolism of a small pool of glutamate that preferentially labels glutamine.