STUDIES ON THE SPECIFICITY OF THE VACCINE EFFECT ELICITED BY INACTIVATED SIMIAN IMMUNODEFICIENCY VIRUS
STUDIES ON THE SPECIFICITY OF THE VACCINE EFFECT ELICITED BY INACTIVATED SIMIAN IMMUNODEFICIENCY VIRUS
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DOI：
10.1089/aid.1993.9.13
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发表时间：
1993-01-01
影响因子：
1.5
通讯作者：
FARRAR, GH
中科院分区：
文献类型：
--
作者：
CRANAGE, MP;POLYANSKAYA, N;FARRAR, GH
灭活的，部分纯化的猿猴免疫缺陷病毒（SIV（mac））保护猕猴从静脉内的挑战与同源和异源株的SIV已在人类细胞上生长，但没有保护对挑战与猴外周血单核细胞生长的SIV（mac）提供。以与SIV（mac）疫苗类似的方式在C8166人T细胞系上制备的人免疫缺陷病毒1型保护猕猴免受人细胞生长的SIV（mac）的攻击。这些结果表明，保护作用可能是由疫苗细胞底物蛋白的异种免疫介导的。所有接种疫苗的猕猴都有抗细胞抗体。主要反应的MHC I类抗原被发现，以及70 kD的蛋白质检测只有在非还原条件下。
Inactivated, partially purified simian immunodefiency virus (SIV(mac)) protected macaques from intravenous challenge with homologous and heterologous strains of SIV that had been grown on human cells but no protection against challenge with monkey peripheral blood mononuclear cell-grown SIV(mac) was afforded. Human immunodeficiency virus type 1 prepared in an analogous way to the SIV(mac) vaccine on the C8166 human T cell line protected macaques against challenge with human cell-grown SIV(mac). These results suggest that protection may be mediated by xenoimmunization with the vaccine cell substrate proteins. All vaccinated macaques had anti-cell antibodies. Major reactivity to MHC class I antigens was found as well as to a 70-kD protein detectable only under nonreducing conditions.