Effects of heat stimulation via microwave applicator on cartilage matrix gene and HSP70 expression in the rabbit knee joint
Effects of heat stimulation via microwave applicator on cartilage matrix gene and HSP70 expression in the rabbit knee joint
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DOI：
10.1002/jor.20421
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发表时间：
2008-01
影响因子：
2.8
通讯作者：
H. Tonomura;Kenji A. Takahashi;O. Mazda;Y. Arai;M. Shin-Ya;A. Inoue;K. Honjo;T. Hojo;J. Imanishi;T. Kubo
中科院分区：
文献类型：
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作者：
H. Tonomura;Kenji A. Takahashi;O. Mazda;Y. Arai;M. Shin-Ya;A. Inoue;K. Honjo;T. Hojo;J. Imanishi;T. Kubo
本研究的目的是研究热刺激对兔关节软骨细胞外基质基因和热休克蛋白70（HSP 70）表达的影响。使用微波（MW）施加器（2.45-GHz，0-80 W）对日本白色家兔的膝关节施加热刺激20 min。8-72小时后，从膝关节取出关节软骨，提取蛋白质和总RNA。作为对照，分析没有热刺激的膝关节。通过真实的时间PCR和Western blotting证实HSP 70的表达。使用真实的时间PCR定量蛋白聚糖核心蛋白（PG）和II型胶原（Col II）的表达，以评估软骨基质代谢。与对照组相比，HSP 70的表达高于40 W的热刺激。PG和Col Ⅱ mRNA表达在热刺激20 W以上时较高，40 W时达到高峰。槲皮素抑制HSP 70的诱导表达后，PG mRNA的表达没有增加。体外微波刺激关节软骨中HSP 70的表达。适当的热刺激可增加细胞外基质基因的表达。© 2007骨科研究学会。出版社：Wiley Periodicals，Inc. J Orthop Res 26：34-41，2008
The objective of this study was to investigate the effects of heat stimulation on the expression of extracellular matrix genes and heat‐shock protein 70 (HSP70) in rabbit articular cartilage in vivo. Heat stimulation was applied to the knee joints of Japanese white rabbits for 20 min using a microwave (MW) applicator (2.45‐GHz, 0–80 W). After 8–72 h, the articular cartilage was removed from the knee joints and proteins and total RNA were extracted. As controls, knee joints without heat stimulation were analyzed. The expression of HSP70 was confirmed by real‐time PCR and Western blotting. The expression of proteoglycan core protein (PG) and type II collagen (Col II) was quantified using real‐time PCR to assess cartilage matrix metabolism. Compared to controls, HSP70 expression was higher with more than 40 W of heat stimulation. The expression of PG and Col II mRNA was higher, with more than 20 W of heat stimulation and peaked with 40 W. When quercetin was used to inhibit the induction of HSP70 expression, PG mRNA expression did not increase. External MW application stimulated HSP70 expression in the articular cartilage in vivo. The expression of extracellular matrix genes was increased by appropriate heat stimulation. © 2007 Orthopaedic Research Society. Published by Wiley Periodicals, Inc. J Orthop Res 26:34–41, 2008