3D NMR experiments for measuring 15N relaxation data of large proteins:: Application to the 44 kDa ectodomain of SIV gp41
3D NMR experiments for measuring 15N relaxation data of large proteins:: Application to the 44 kDa ectodomain of SIV gp41
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DOI：
10.1006/jmre.1998.1583
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发表时间：
1998-12-01
影响因子：
2.2
通讯作者：
Clore, GM
中科院分区：
文献类型：
--
作者：
Caffrey, M;Kaufman, J;Clore, GM
一套三维NMR实验测量N-15-{H-1} NOE，N-15 T-1，和N-15 T-1 rho值的大蛋白质，均匀标记的N-15和C-13，提出。这些实验设计用于在2D H-1-N-15 HSQC光谱中表现出广泛光谱重叠的蛋白质。脉冲序列很容易适用于全氘样品，这增加了光谱分辨率和信噪比，从而允许蛋白质动力学的表征扩展到更大的蛋白质系统。在SIV gp 41的44 kDa胞外域的全氘代C-13/N-15标记样品上证明了脉冲序列的应用。
A suite of 3D NMR experiments for measuring N-15-{H-1} NOE, N-15 T-1, and N-15 T-1 rho values in large proteins, uniformly labeled with N-15 and C-13, is presented. These experiments are designed for proteins that exhibit extensive spectral overlap in the 2D H-1-N-15 HSQC spectrum. The pulse sequences are readily applicable to perdeuterated samples, which increases the spectral resolution and signal-to-noise ratio, thereby permitting the characterization of protein dynamics to be extended to larger protein systems. Application of the pulse sequences is demonstrated on a perdeuterated C-13/N-15-labeled sample of the 44 kDa ectodomain of SIV gp41.