Addition of genistein to the fermentation process reduces citrinin production by Monascus via changes at the transcription level
Addition of genistein to the fermentation process reduces citrinin production by Monascus via changes at the transcription level
复制标题
在发酵过程中添加染料木素可通过转录水平的变化减少红曲菌产生的柑橘素
DOI：
10.1016/j.foodchem.2020.128410
复制
发表时间：
2021-05-01
期刊：
影响因子：
8.8
通讯作者：
Li, Xiujiang
中科院分区：
文献类型：
--
作者：
Ouyang, Wanbao;Liu, Xin;Li, Xiujiang
传统上用于亚洲各行业的蒙丹酸在发酵过程中产生若干次级代谢物，包括桔霉素，这种毒素的影响限制了蒙丹酸行业的发展。我们以前已经发现，添加2.0 g/L染料木黄酮到Monoclonal培养基中可使桔霉素产量降低约80%。在这里，我们探讨了染料木素影响桔霉素生产的分子机制。我们对Monoclonal基因组进行了测序，并对染料木黄酮治疗组和未治疗组进行了转录组分析。两组比较，下调基因378个，上调基因564个。在后者中，我们进一步研究了与桔霉素生物合成相关的基因，并使用定量实时聚合酶链反应（qRT-PCR）对其进行定量。基因orf 5，pksCT，orf 3，orf 1，orf 6，和ctnE显着下调，表明染料木素除了确实影响桔霉素的合成。我们的研究结果可能奠定了基础，在蒙乃尔发酵工业的实质性改进。
Monascus, which is traditionally used in various Asian industries, produces several secondary metabolites during the fermentation process, including citrinin, a toxin whose impact limits the development of the Monascus industry. We have previously found that the addition of 2.0 g/L genistein to Monascus medium reduces citrinin production by approximately 80%. Here, we explored the molecular mechanisms whereby genistein affects citrinin production. We sequenced the Monascus genome and performed transcriptome analysis on genisteintreated and-untreated groups. Comparison between the two groups showed 378 downregulated and 564 up regulated genes. Among the latter, we further examined the genes related to citrinin biosynthesis and quantified them using quantitative real-time polymerase chain reaction (qRT-PCR). Genes orf5, pksCT, orf3, orf1, orf6, and ctnE were significantly downregulated, demonstrating that genistein addition indeed affects citrinin synthesis. Our results may lay the groundwork for substantial improvements in the Monascus fermentation industry.