Induction of apatite by the cooperative effect of amelogenin and the 32-kDa enamelin
Induction of apatite by the cooperative effect of amelogenin and the 32-kDa enamelin
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DOI：
10.1177/154405910408300402
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发表时间：
2004-04-01
影响因子：
7.6
通讯作者：
Moradian-Oldak, J
中科院分区：
文献类型：
--
作者：
Bouropoulos, N;Moradian-Oldak, J
细胞外基质蛋白被认为在控制釉质形成过程中羟基磷灰石晶体的成核、生长和组织中起重要作用。釉原蛋白和32 kDa的釉蛋白对磷灰石成核的影响进行了研究，通过稳态凝胶扩散装置含有10%明胶凝胶加载0，0.75%，和1.5%（w/w）的本地猪釉原蛋白。结果发现，诱导时间为羟基磷灰石沉淀强烈增加的存在下的釉原蛋白，这表明磷灰石成核的抑制作用。向10%明胶中加入18 μ g/mL的32-kDa釉蛋白也会抑制成核。值得注意的是，在含有1.5%釉原蛋白的凝胶中加入18和80 μ g/mL的32-kDa釉蛋白以剂量依赖性方式加速成核过程。我们的观察结果强烈表明，32 kDa的釉蛋白和釉原蛋白合作，以促进磷灰石晶体的成核，并提出了一个可能的新的机制，矿物成核过程中釉质生物矿化。
Extracellular matrix proteins are considered to play essential roles in controlling the nucleation, growth, and organization of hydroxyapatite crystals during enamel formation. The effects of amelogenin and the 32-kDa enamelin proteins on apatite nucleation were investigated by a steady-state gel diffusion device containing 10% gelatin gels loaded with 0, 0.75%, and 1.5% (w/w) native porcine amelogenins. It was found that the induction time for hydroxyapatite precipitation was strongly increased by the presence of amelogenins, suggesting an inhibitory effect of apatite nucleation. Addition of 18 mug/mL of 32-kDa enamelin to 10% gelatin also caused inhibition of nucleation. Remarkably, addition of 18 and 80 mug/mL of 32-kDa enamelin in gels containing 1.5% amelogenin accelerated the nucleation process in a dose-dependent manner. Our observations strongly suggest that the 32-kDa enamelin and amelogenins cooperate to promote nucleation of apatite crystals and propose a possible novel mechanism of mineral nucleation during enamel biomineralization.