A new method for excitation-contraction uncoupling in frog skeletal muscle.
A new method for excitation-contraction uncoupling in frog skeletal muscle.
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DOI：
10.1083/jcb.78.3.782
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发表时间：
1978-09
影响因子：
7.8
通讯作者：
Escalona de Motta, G
中科院分区：
文献类型：
--
作者：
del Castillo, J;Escalona de Motta, G
通过将制剂浸入含有 1.5 或 2.0 M 甲酰胺的林格溶液中 15--20 分钟，可以将青蛙缝匠肌纤维的机械活动与其表面膜的电活动解耦。当将肌肉转移到正常的青蛙林格溶液中时，这种解偶联不会逆转。甲酰胺不影响坐骨神经分支的电活动，并且可以从未耦合的肌肉记录终板电位和微型终板电位。长时间接触甲酰胺，超过麻痹所需的时间，会导致神经肌肉阻滞。
The mechanical activity of frog sartorius muscle fibers can be uncoupled from the electrical activity of their surface membranes by immersing the preparation in Ringer solution containing either 1.5 or 2.0 M of formamide for 15--20 min. This uncoupling is not reversed when the muscle is transferred to normal frog Ringer solution. Formamide does not affect the electrical activity of the sciatic nerve branch, and both endplate potentials and miniature endplate potentials may be recorded from the uncoupled muscles. Prolonged exposure to formamide, beyond the time needed to paralyze, causes neuromuscular block.