The tyrosine-sulfated peptide receptors PSKR1 and PSY1R modify the immunity of Arabidopsis to biotrophic and necrotrophic pathogens in an antagonistic manner
The tyrosine-sulfated peptide receptors PSKR1 and PSY1R modify the immunity of Arabidopsis to biotrophic and necrotrophic pathogens in an antagonistic manner
复制标题
DOI：
10.1111/tpj.12050
复制
发表时间：
2013-02-01
期刊：
影响因子：
7.2
通讯作者：
Kemmerling, Birgit
中科院分区：
文献类型：
--
作者：
Mosher, Stephen;Seybold, Heike;Kemmerling, Birgit
酪氨酸硫酸化肽PSKa和PSY 1与特异性富含亮氨酸的重复序列表面受体激酶结合并控制植物细胞增殖。在反向遗传筛选中，我们确定了植物磺因子（PSK）受体PSKR 1作为植物防御的重要组成部分。PSKR 1中的多个独立的功能丧失突变体对活体营养细菌更具抗性，在感染细菌病原体假单胞菌致病变种后显示出增强的病原体相关分子模式反应和较少的损伤形成。番茄DC 3000。相比之下，pskr 1突变体更容易受到十字花科链格孢的坏死型真菌感染，表现出更多的病变形成和真菌生长，这在野生型植物上没有观察到。增强水杨酸和茉莉酸响应的突变体，表明PSKR 1抑制水杨酸依赖的防御反应的拮抗作用的生物营养型和坏死营养型病原体的阻力。对三个旁系同源基因PSKR 1、PSKR 2和PSY 1受体（PSY 1 R）中的单个和多个突变的详细分析确定，PSKR 1和PSY 1 R，而不是PSKR 2，对植物免疫具有部分冗余的影响。在动物和植物中，肽硫酸化由酪蛋白磺基转移酶（TPST）催化。缺乏TPST的突变体表现出对细菌感染的抗性增加和对真菌感染的易感性增加，模拟三重受体突变体表型。在tpst-1突变体中用PSKa进行的饲养实验部分恢复了防御相关的表型，表明PSKa肽的感知对植物防御有直接影响。这些结果表明，PSKR亚家族整合了由硫酸化肽介导的生长促进和防御信号，并调节细胞可塑性，以适应环境变化。
The tyrosine-sulfated peptides PSKa and PSY1 bind to specific leucine-rich repeat surface receptor kinases and control cell proliferation in plants. In a reverse genetic screen, we identified the phytosulfokine (PSK) receptor PSKR1 as an important component of plant defense. Multiple independent loss-of-function mutants in PSKR1 are more resistant to biotrophic bacteria, show enhanced pathogen-associated molecular pattern responses and less lesion formation after infection with the bacterial pathogen Pseudomonas syringae pv. tomato DC3000. By contrast, pskr1 mutants are more susceptible to necrotrophic fungal infection with Alternaria brassicicola, show more lesion formation and fungal growth which is not observed on wild-type plants. The antagonistic effect on biotrophic and necrotrophic pathogen resistance is reflected by enhanced salicylate and reduced jasmonate responses in the mutants, suggesting that PSKR1 suppresses salicylate-dependent defense responses. Detailed analysis of single and multiple mutations in the three paralogous genes PSKR1, -2 and PSY1-receptor (PSY1R) determined that PSKR1 and PSY1R, but not PSKR2, have a partially redundant effect on plant immunity. In animals and plants, peptide sulfation is catalyzed by a tyrosylprotein sulfotransferase (TPST). Mutants lacking TPST show increased resistance to bacterial infection and increased susceptibility to fungal infection, mimicking the triple receptor mutant phenotypes. Feeding experiments with PSKa in tpst-1 mutants partially restore the defense-related phenotypes, indicating that perception of the PSKa peptide has a direct effect on plant defense. These results suggest that the PSKR subfamily integrates growth-promoting and defense signals mediated by sulfated peptides and modulates cellular plasticity to allow flexible adjustment to environmental changes.