The role of factor XI in the coagulant activity of platelets.
The role of factor XI in the coagulant activity of platelets.
复制标题
XI 因子在血小板凝血活性中的作用。
DOI：
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发表时间：
1977
期刊：
影响因子：
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通讯作者：
R. Muntz
中科院分区：
文献类型：
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作者：
J. Connellan;P. Castaldi;R. Muntz
洗涤的血小板通过冷冻和解冻而破裂；只有在高岭土存在的情况下，才会释放凝血剂活性，以纠正凝血因子XI(FXI)缺乏的血浆的凝血时间。以类似方式治疗的FXI缺陷患者的血小板也释放凝血活性，该活性可被Sephoseheparin吸收，并与血浆FXI类似地洗脱。在存在和不存在针对纯化的FXI和FXII的抗体的情况下，胶原被用来在缺乏血小板的血浆(PPP)和富含血小板的血浆(PRP)中诱导凝血活性。FXII抗体的存在对PRP诱导的活性影响不大。然而，FXI抗体的存在消除了PPP和PRP之间的差异。当FXI缺陷的PRP与胶原蛋白孵育时，可诱导其活性，而与PPP孵育时则不诱导。一种胶原蛋白不能诱导凝血活性。
Washed platelets were ruptured by freezing and thawing; a coagulant activity was released which would correct the clotting time of factor XI (FXI)-deficient plasma only in the presence of kaolin. Platelets from a FXI-deficient patient treated in a similar fashion also released a coagulant activity which could be absorbed onto Sepharose-heparin and eluted similarly to plasma FXI. Collagen was employed to induce a coagulant activity in platelet-poor plasma (PPP) and platelet-rich plasma (PRP) in the presence and absence of antibodies developed to purified FXI and FXII. The presence of FXII antibody had little effect on the activity induced in PRP. However, the presence of FXI antibody eliminated the difference between PPP and PRP. An activity was induced when FXI-deficient PRP was incubated with collagen and none with PPP. One type of collagen failed to induce a coagulant activity.