Localized proton magnetic resonance spectroscopy of lipids in adipose tissue at high spatial resolution in mice in vivo
Localized proton magnetic resonance spectroscopy of lipids in adipose tissue at high spatial resolution in mice in vivo
复制标题
DOI：
10.1194/jlr.d700024-jlr200
复制
发表时间：
2008-02-01
影响因子：
6.5
通讯作者：
Pietzsch, Jens
中科院分区：
文献类型：
--
作者：
Strobel, Klaus;van den Hoff, Joerg;Pietzsch, Jens
我们描述了一种定位的质子磁共振波谱(H-1-MRS)方法，用于在体测量小鼠脂肪组织中非常小的体素(1.5 mm x 1.5 mm 3 1.5 mm)的脂肪成分。该方法使用局域点分辨光谱从腹内白色脂肪组织(WAT)和棕色脂肪组织(BAT)沉积的体素中收集H-1光谱。频域光谱的非线性最小二乘拟合允许准确地计算饱和、单不饱和和多不饱和脂肪酸的相对量。所有的光谱数据都经过了自旋-自旋弛豫校正。数据显示，NMRI小鼠的BAT与NMRI nu/nu小鼠的BAT除单不饱和脂肪酸组成(Fm)外，其余各方面均有显著差异；对于Wat，只有Fm不同。NMRI小鼠的BAT和WAT在饱和和二不饱和脂肪酸的数量上有所不同。该方法为研究体内肥胖相关疾病的发生、发展和表现过程中小动物模型的脂代谢提供了一个潜在的工具。我们的结果清楚地表明，在体素大小降至3.4ml的高空间分辨率下，体内脂肪组织的H-1-MRS定位是可能的。
We describe a localized proton magnetic resonance spectroscopy (H-1-MRS) method for in vivo measurement of lipid composition in very small voxels (1.5 mm x 1.5 mm 3 1.5 mm) in adipose tissue in mice. The method uses localized point-resolved spectroscopy to collect H-1 spectra from voxels in intra-abdominal white adipose tissue (WAT) and brown adipose tissue (BAT) deposits. Nonlinear least-squares fits of the spectra in the frequency domain allow for accurate calculation of the relative amount of saturated, monounsaturated, and polyunsaturated fatty acids. All spectral data are corrected for spin-spin relaxation. The data show BAT of NMRI mice to be significantly different from BAT of NMRI nu/nu mice in all aspects except for the fraction of monounsaturated fatty acids (FM); for WAT, only the FM is different. BAT and WAT of NMRI mice differ in the amount of saturated and diunsaturated fatty acids. This method provides a potential tool for studying lipid metabolism in small animal models of disease during the initiation, progression, and manifestation of obesity-related disorders in vivo. Our results clearly demonstrate that localized H-1-MRS of adipose tissue in vivo is possible at high spatial resolution with voxel sizes down to 3.4 ml.