Photodynamic Hyperthermal Therapy with Indocyanine Green (ICG) induces Apoptosis and Cell Cycle Arrest in B16F10 Murine Melanoma Cells
Photodynamic Hyperthermal Therapy with Indocyanine Green (ICG) induces Apoptosis and Cell Cycle Arrest in B16F10 Murine Melanoma Cells
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DOI：
10.1292/jvms.11-0464
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发表时间：
2012-05-01
影响因子：
1.2
通讯作者：
Okamoto, Yoshiharu
中科院分区：
文献类型：
--
作者：
Radzi, Rozanaliza;Osaki, Tomohiro;Okamoto, Yoshiharu
我们研究了光动力热疗（PHT），这是光动力疗法（PDT）和热疗（HT）的组合，对小鼠黑色素瘤B16 F10细胞的凋亡和细胞周期进程的影响。流式细胞仪检测凋亡细胞百分率，采用异硫氰酸荧光素（FITC）标记的Annexin V和碘化丙啶（PI）双重染色。流式细胞仪PI染色分析细胞周期。Western blotting检测细胞周期蛋白和热休克蛋白70（Hsp 70）的表达。PHT诱导B16 F10细胞死亡，并且PHT介导的细胞凋亡在体外急性且持续地发生。我们的研究表明，使用吲哚菁绿色（ICG）和近红外（NIR）光源的PHT诱导B16 F10细胞凋亡和G 0/G1期阻滞。
We examined the effects of photodynamic hyperthemal therapy (PHT), which is a combination of photodynamic therapy (PDT) and hyperthermia (HT), on the apoptosis and cell cycle progression of murine melanoma B16F10 cells. The percentage of apoptotic cell was determined by flow cytometry using fluorescein isothiocyanate (FITC)-conjugated Annexin V and propidium iodide (PI) double staining. The cell cycle analysis was performed by PI staining with flow cytometry. The expression of cycl ins and heat shock protein 70 (Hsp70) were examined by a Western blotting analysis. PHT induces death in B16F10 cells, and PHT-mediated apoptosis occurred acutely and persistently in vitro. Our study demonstrated that PHT using indocyanine green (ICG) and near infrared (NIR) light source induces apoptosis and G0/G1 cell cycle arrest in the B16F10 cells.