Expression of Osteoclastogenesis Inducers in a Tissue Model of Periodontal Ligament under Compression
Expression of Osteoclastogenesis Inducers in a Tissue Model of Periodontal Ligament under Compression
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牙周膜受压组织模型中破骨细胞生成诱导物的表达
DOI：
10.1177/0022034510385237
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发表时间：
2011-01-01
影响因子：
7.6
通讯作者：
Zhao, Z. -H.
中科院分区：
文献类型：
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作者：
Li, Y.;Zheng, W.;Zhao, Z. -H.
人们对开发新的体外组织模型越来越感兴趣。本研究通过在多孔聚乳酸-羟基乙酸支架上对人牙周膜细胞进行三维培养，建立牙周膜组织模型。MTT试验和各种显微镜观察证实了模型的生长。在5 ~ 35 g/cm(2)的静压缩作用6小时后，模型中RANKL mRNA的表达量在25 g/cm(2)的压力下显著上调。在类似于25 g/cm(2)的静态压缩6小时后，模型中潜在的破骨细胞生成诱导因子PTHrP、IL-11、IL-8和FGF-2的mRNA表达显著上调，并通过人体PDL的体内压缩进一步验证了这一点。然而，当用人牙龈成纤维细胞代替模型中的PDL细胞时，几乎没有破骨细胞生成诱导因子被压缩上调。该组织模型可作为研究PDL力学反应的有效工具。缩写：牙周韧带，PDL；牙周韧带细胞（pdlc）；聚乳酸-乙醇酸；正畸牙齿移动；细胞外基质
There is increasing interest in the development of new in vitro tissue models. In this study, a tissue model of periodontal ligament (PDL) was established by 3-D-culturing human PDL cells in a thin sheet of porous poly (lactic-co-glycolic acid) scaffold. Growth of the model was evidenced by MTT assay and various microscopies. After being subjected to static compression of 5 similar to 35 g/cm(2) for 6 hrs, the RANKL mRNA expression was significantly up-regulated by force > 25 g/cm(2) in the model. After being subjected to static compression of 25 g/cm(2) for 6 similar to 72 hrs, the mRNA expression of PTHrP, IL-11, IL-8, and FGF-2, potential osteoclastogenesis inducers, was significantly upregulated in the model, which was further verified by the compression of human PDL in vivo. However, when human gingival fibroblasts were substituted for PDL cells in the model, almost no osteoclastogenesis inducers were up-regulated by compression. This tissue model can serve as an effective tool for the study of PDL mechanoresponse. Abbreviations: periodontal ligament, PDL; periodontal ligament cells, PDLCs; poly (lacticco-glycolic acid), PLGA; orthodontic tooth movement, OTM; extracellular matrix, ECM.