Porphyromonas gingivalis culture supernatants differentially regulate Interleukin-1β and Interleukin-18 in human monocytic cells
Porphyromonas gingivalis culture supernatants differentially regulate Interleukin-1β and Interleukin-18 in human monocytic cells
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DOI：
10.1016/j.cyto.2008.11.005
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发表时间：
2009-02-01
期刊：
影响因子：
3.8
通讯作者：
Bostanci, N.
中科院分区：
文献类型：
--
作者：
Hamedi, M.;Belibasakis, G. N.;Bostanci, N.
牙龈卟啉单胞菌是慢性牙周炎的主要细菌种类，慢性牙周炎是一种以牙齿支持组织炎症性破坏为特征的疾病。其主要毒力因子是脂多糖（LPS）和牙龈蛋白酶（一组半胱氨酸蛋白酶）。白细胞介素 (IL)-18 是一种有效的促炎细胞因子，其结构与 IL-1 β 相似。本研究旨在探讨牙龈卟啉单胞菌是否调节单核细胞中的 IL-1 β 和 IL-18。用牙龈卟啉单胞菌培养上清液攻击 Monomac-6 细胞。实时定量PCR和ELISA分别用于研究IL-1β和IL-18 mRNA表达和蛋白质分泌。牙龈卟啉单胞菌增强了IL-1β和IL-18 mRNA表达，前者被诱导较早，但短暂。 IL-18 上调不受牙龈卟啉单胞菌热灭活或其牙龈蛋白酶化学抑制的影响，而两种处理均导致 IL-1 β 表达减少 50%。纯化的牙龈曲霉 LPS 增强了 IL-1β 和 IL-18 的表达。然而，仅检测到IL-1β的分泌，而未检测到IL-18的分泌，并且牙龈卟啉单胞菌上调了IL-1β的分泌。总之，虽然IL-1β和IL-18属于同一细胞因子家族，但它们的基因表达和分泌在人单核细胞中针对牙龈卟啉单胞菌的反应而受到差异性调节。因此，IL-1家族的细胞因子可能通过不同的途径参与牙周炎的复杂发病机制。 (C) 2008 Elsevier Ltd. 保留所有权利。
Porphyromonas gingivalis is a major bacterial species implicated in chornic periodontitis, a disease characterized by inflammatory destruction of the tooth supporting tissues. Its main virulence factors are lipopolysaccharide (LPS) and gingipains, a group of cysteine proteinases. Interleukin (IL)-18 is a potent pro-inflammatory cytokine with structural similarities to IL-1 beta. This study aimed to investigate if P.gingivalis regulates IL-1 beta and IL-18 in monocytic cells. Monomac-6 cells were challenged with P. gingivalis culture supernatants. Quantitative real-time PCR and ELISA were used to investigate IL-1 beta and IL-18 mRNA expression and protein secretion, respectively. P. gingivalis enhanced IL-1 beta and IL-18 mRNA expression, the former being induced earlier, but transiently. IL-18 up-regulation was not affected by P. gingivalis heat-inactivation or chemical inhibition of its gingipains, whereas both treatments resulted in 50% reduction of IL-1 beta expression. Purified A gingivalis LPS enhanced both IL-1 beta and IL-18 expression. However, only IL-1 beta, but not IL-18, secretion was detected, and was up-regulated by P. gingivalis. In conclusion, although IL-1 beta and IL-18 belong to the same cytokine family, their gene expression and secretion are differentially regulated in human monocytic cells in response to P. gingivalis. Therefore, cytokines of the IL-1 family may participate via different pathways in the complex pathogenesis of periodontitis. (C) 2008 Elsevier Ltd. All rights reserved.