Comparative proteomics of spinal cords of rat fetuses with spina bifida aperta
Comparative proteomics of spinal cords of rat fetuses with spina bifida aperta
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脊柱裂大鼠胎儿脊髓的比较蛋白质组学
DOI：
10.1016/j.jprot.2011.09.006
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发表时间：
2011-12-21
影响因子：
3.3
通讯作者：
Yuan, Zhengwei
中科院分区：
文献类型：
--
作者：
Fan, Yang;Wang, Lili;Yuan, Zhengwei
神经管缺陷（NTDs）是中枢神经系统复杂的先天性异常，全球患病率为5 / 10,000。然而，目前治疗被忽视热带病的方法并不令人满意。神经系统并发症仍然是治疗的主要问题。子宫内未显露的神经组织的原发缺损或损伤可能导致神经功能障碍。然而，未发现的神经组织的病理变化尚未描述。在这里，我们对全反式维甲酸诱导的先天性脊柱裂大鼠胎儿的脊髓进行了比较蛋白质组学研究。脊髓蛋白进行二维凝胶电泳，然后进行质谱鉴定。我们鉴定出13种蛋白在正常脊髓和腹裂脊髓之间有差异表达。据报道，这些鉴定的蛋白质参与信号转导，细胞粘附和迁移，蛋白质折叠和凋亡。我们用免疫印迹法对鉴定出的4个蛋白进行了确认，并用实时荧光定量PCR法检测了它们的mRNA水平。这是第一个比较蛋白质组学的大鼠胎儿脊柱裂。我们证明了蛋白质的改变反映了未发现的神经组织的病理情况，这可能有助于改善NTDs的治疗。(C) 2011 Elsevier B.V.版权所有
Neural tube defects (NTDs) are complex congenital anomalies of the central nervous system, with a prevalence of 5 per 10,000 worldwide. However, current therapeutics for NTDs are unsatisfactory. The neurological complications remain the main problem for therapy. Neurological dysfunction could result from the primary defect or injuries to the uncovered neural tissue in the uterus. However, the pathological changes in the uncovered neural tissue have not been described. Here, we present our comparative proteomics study of the spinal cord from rat fetuses with all-trans retinoic-acid-induced spina bifida aperta. Proteins from spinal cords were subjected to 2-D gel electrophoresis, then protein identification by mass spectrometry. We identified 13 proteins with differential expression between normal spinal cords and those with spina bifida aperta. These identified proteins were reported to be involved in signal transduction, cell adhesion and migration, protein folding and apoptosis. We confirmed 4 identified proteins by immunoblot analysis and assessed their mRNA levels by quantitative real-time PCR. This is the first comparative proteomics of spinal cords from rat fetuses with spina bifida aperta. We demonstrate protein alterations that reflect the pathological situation of the uncovered neural tissue, which may help improve the treatment of NTDs. (C) 2011 Elsevier B.V. All rights reserved.