Single CNS neurons link both central motor and card iosympathetic systems: A double-virus tracing study
Single CNS neurons link both central motor and card iosympathetic systems: A double-virus tracing study
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DOI：
10.1016/s0306-4522(02)00997-1
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发表时间：
2003-01-01
期刊：
影响因子：
3.3
通讯作者：
Loewy, AD
中科院分区：
文献类型：
--
作者：
Krout, KE;Mettenleiter, TC;Loewy, AD
两个解剖学实验被用来验证这样一个假设，即单个CNS神经元连接着调节躯体运动和交感系统的中枢区域。首先，将逆行神经元示踪霍乱毒素β亚基注射到丘脑束旁外侧核，该区域既投射到运动皮质，也投射到纹状体。几天后，在同一只大鼠的星状神经节进行了第二次逆行跨神经元示踪剂伪狂犬病病毒(PRV)注射，该星状神经节为心脏提供主要的交感神经供应。用免疫组织化学方法，我们证明了桥脑脚被盖核(PPN)的胆碱能神经元与这两个系统相连。第二个实验使用两个Bartha PRV同基因株作为双跨神经元示踪剂。一种病毒含有绿色荧光蛋白(GFP)基因，另一种病毒含有β-半乳糖苷酶(β-Gal)基因。星状神经节注射GFP-PRV，初级运动皮质注射β-Gal-PRV。下丘脑外侧区(50%含增食欲素)和PPN区(约95%为胆碱能)可见双标记神经元。其他双标细胞还见于颞叶深部(杏仁海马区和外侧内嗅皮层)、下丘脑后部、结节乳头腹侧核、蓝斑、被盖外侧核、中脑导水管周围灰质、中缝背核和孤束核。这些结果表明，这些假定的指挥神经元整合了躯体运动和心交感神经功能，并可能影响不同的行为(即唤醒、睡眠和/或运动)。(C)2003年IBRO。爱思唯尔科学有限公司出版。版权所有。
Two anatomical experiments were performed to test the hypothesis that single CNS neurons link the central areas that regulate the somatomotor and sympathetic systems. First, the retrograde neuronal tracer cholera toxin beta-subunit was injected into the lateral parafascicular thalamic nucleus, a region that projects to both the motor cortex and striatum. Several days later, a second injection of the retrograde transneuronal tracer, pseudorabies virus (PRV), was made in the same rats in the stellate ganglion, which provides the main sympathetic supply to the heart. Using immunohistochemical methods, we demonstrate that the cholinergic neurons of the pedunculopontine tegmental nucleus (PPN) are connected to both systems. The second experiment used two isogenic strains of Bartha PRV as double transneuronal tracers. One virus contained the unique gene for green fluorescent protein (GFP) and the other had the unique gene for P-galactosidase (beta-gal). GFP-PRV was injected in the stellate ganglion and beta-gal-PRV was injected into the primary motor cortex. Double-labeled neurons were found in the lateral hypothalamic area (50% contained orexin) and PPN (approximately 95% were cholinergic). Other double-labeled neurons were identified in the deep temporal lobe (viz., amygdalohippocampal zone and lateral entorhinal cortex), posterior hypothalamus, ventral tuberomammillary nucleus, locus coeruleus, laterodorsal tegmental nucleus, periaqueductal gray matter, dorsal raphe nucleus, and nucleus tractus solitarius. These results suggest these putative command neurons integrate the somatomotor and cardiosympathetic functions and may affect different behaviors (viz., arousal, sleep, and/or locomotion). (C) 2003 IBRO. Published by Elsevier Science Ltd. All rights reserved.