Genetic characterization of a new splice variant of the β2 subunit of the voltage-dependent calcium channel
Genetic characterization of a new splice variant of the β2 subunit of the voltage-dependent calcium channel
复制标题
电压依赖性钙通道β2亚基新剪接变体的遗传特征
DOI：
--
复制
发表时间：
2003
影响因子：
4.3
通讯作者：
T. Iijima
中科院分区：
文献类型：
--
作者：
M. Murakami;M. Aoyama;Takashi Suzuki;H. Sasano;S. Nakayama;T. Iijima
本研究报道了电压依赖性钙通道β2亚基（β2g）的一种新的剪接变体。这种变体由β2a亚基的保守氨基末端序列组成，但缺乏β亚基相互作用结构域（BID），这被认为是与α1亚基相互作用所必需的。基因结构分析表明，该基因由13个翻译外显子组成，分布在107 kb的基因组中。β2亚基的基因结构在外显子-内含子组织方面与鼠β3和人β4亚基相似。电生理评价表明β2a和β2g以不同的方式影响通道特性。β2a亚基增加了通道的峰值电流，但不能增加通道的失活，而β2g亚基对通道的失活和峰值电流无明显影响。其他β亚基如β3和β4显著增加峰电流并加速电流失活。
This study reports a novel splice variant form of the voltage-dependent calcium channel β2 subunit (β2g). This variant is composed of the conserved amino-terminal sequences of the β2a subunit, but lacks the β-subunit interaction domain (BID), which is thought essential for interactions with the α1 subunit. Gene structure analysis revealed that this gene was composed of 13 translated exons spread over 107 kb of the genome. The gene structure of the β2 subunit was similar in exon-intron organization to the murine β3 and human β4 subunits. Electrophysiological evaluation revealed that β2a and β2g affected channel properties in different ways. The β2a subunit increased the peak amplitude, but failed to increase channel inactivation, while β2g had no significant effects on either the peak current amplitude or channel inactivation. Other β subunits, such as β3 and β4, significantly increased the peak current and accelerated current inactivation.
DOI：
10.1073/pnas.95.8.4690
发表时间：
1998-04-14
影响因子：
11.1
作者：
Qin, N;Platano, D;Birnbaumer, L
通讯作者：
Birnbaumer, L
第三个钙通道β亚基的克隆和表达。
DOI：
--
发表时间：
1993
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Castellano,A;Wei,X;Birnbaumer,L;Perez-Reyes,E
通讯作者：
Perez-Reyes,E