A novel assay for quantifying the number of plasmids encapsulated by polymer nanoparticles.

A novel assay for quantifying the number of plasmids encapsulated by polymer nanoparticles.
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DOI:
10.1002/smll.201101718
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发表时间:
2012-02-06
期刊:
影响因子:
13.3
通讯作者:
Green, Jordan J.
Green, Jordan J.
中科院分区:
材料科学1区
文献类型:
--
作者:
Bhise, Nupura S.;Shmueli, Ron B.;Gonzalez, Jose;Green, Jordan J.

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Polymeric nanoparticles are promising for gene therapy and stem cell reprogramming using non-viral vectors. A novel assay utilizing nanoparticle tracking analysis (left) is developed here to easily quantify the number of plasmids within polymeric nanoparticles while in aqueous solution. Particles effective at co-transfecting primary human fibroblasts (right) are ~100 nm in diameter and contain ~100 plasmids per particle.
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