Assays on DNA Damage and Repair in CLL
Assays on DNA Damage and Repair in CLL
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DOI：
10.1007/978-1-4939-8876-1_12
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发表时间：
2019-01-01
期刊：
影响因子：
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通讯作者：
Sampath, Deepa
中科院分区：
文献类型：
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作者：
Lai, Tzung-Huei;Sampath, Deepa
测量DNA损伤和修复的试验对于评估治疗药物损伤DNA的程度以及确定DNA修复是否可以限制化疗的毒性至关重要。本指南中描述的COMET试验应有助于读者评估由化疗药物引起的单链和双链断裂，并监测细胞修复这种损伤的能力。所描述的EJDR分析是评估药物和DNA修复蛋白调节DNA修复能力的有价值的工具。最后，描述的免疫荧光分析应该能够准确评估DNA损伤和修复动力学，通过γ - h2ax焦点测量。该方法也可用于研究CLL细胞中特异性DNA损伤和修复蛋白的募集机制。
Assays that measure DNA damage and repair are critical in evaluating the extent to which therapeutic agents damage DNA and in identifying whether DNA repair can limit the toxicity of chemotherapy. The COMET assays described in this guide should help readers evaluate single and double-strand breaks cause by chemotherapeutic agents and also monitor the ability of the cells to repair such damage. The EJDR assay described is a valuable tool to assess the ability of drugs and DNA repair proteins to modulate DNA repair capacity. Finally, the immunofluorescence assay described should allow accurate assessments of DNA damage and the kinetics of repair as measured by gamma-H2AX foci. This procedure can also be used to mechanistically investigate the recruitment of specific DNA damage and repair proteins in CLL cells.